| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eggers, Kaylee J (11)
|
NCRMY |
2:26.87 |
2:31.61 |
4.74 |
| 1 |
Sikes, Katherine B (12)
|
NCHAC |
2:50.06 |
2:32.17 |
-17.89 |
| 1 |
Russell, Leilani M (12)
|
NCLOY |
2:41.32 |
2:39.72 |
-1.60 |
| 1 |
Seifert, Camille I (11)
|
NCLOY |
|
2:57.40 |
- |
| 2 |
Bellissimo, Sophia G (11)
|
NCLOY |
2:44.67 |
2:44.15 |
-0.52 |
| 2 |
Buchholtz, Chloe E (12)
|
NCACCH |
2:50.59 |
2:48.02 |
-2.57 |
| 2 |
Challa, Mia K (11)
|
NCHAC |
3:10.99 |
3:03.19 |
-7.80 |
| 3 |
Bush, Savanna N (12)
|
NCLOY |
3:00.69 |
2:49.00 |
-11.69 |
| 3 |
Brewer, Kamryn B (12)
|
NCLOY |
3:07.54 |
3:03.80 |
-3.74 |
| 4 |
Hettinger, Emma G (12)
|
NCLOY |
3:18.80 |
3:04.41 |
-14.39 |
| 5 |
Carter, Braylee M (12)
|
NCYBAC |
|
3:07.99 |
- |
| 6 |
Allred, Grace E (12)
|
NCHAC |
4:00.99 |
3:33.46 |
-27.53 |
|
Pinnell, Anneliese S (11)
|
NCHAC |
2:58.94 |
NS |
- |