| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnson, Emory R (8)
|
NCFAST |
24.00 |
26.04 |
2.04 |
| 2 |
Carter, Addyson G (8)
|
NCRACY |
26.27 |
26.51 |
0.24 |
| 3 |
Milligan, Sarah M (7)
|
NCRMY |
26.76 |
29.25 |
2.49 |
| 4 |
Challa, Maya I (8)
|
NCHAC |
|
30.25 |
- |
| 5 |
Hoos, Caroline J (8)
|
NCACCH |
|
30.62 |
- |
| 6 |
James, Mikayla I (8)
|
NCHAC |
|
32.68 |
- |
| 7 |
Grau, Taylor M (7)
|
NCNSS |
|
33.53 |
- |
| 8 |
Stephens, Emma W (8)
|
NCACCH |
|
35.63 |
- |
| 9 |
Kibbe, Elizabeth E (6)
|
NCRMY |
36.45 |
37.38 |
0.93 |
| 10 |
Wallace, Celeste E (6)
|
NCCAST |
|
42.86 |
- |
| 11 |
Tisch, Lily C (5)
|
NCCKS |
|
56.21 |
- |
|
Rodriguez, Sophia E (6)
|
NCYBAC |
42.25 |
DQ |
- |
|
Kenney, Abigail C (6)
|
NCCAST |
42.31 |
DQ |
- |
|
Dew, Elisa E (7)
|
NCHAC |
|
DQ |
- |
|
Herlocker, Patterson L (6)
|
NCRACY |
|
DQ |
- |