| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Feenstra, Bo R (8)
|
MIWMS |
|
44.09 |
- |
| 2 |
Gorman, Tupper C (8)
|
MIEGRA |
|
44.33 |
- |
| 3 |
Flermoen, Bennett T (8)
|
MIEGRA |
44.45 |
46.35 |
1.90 |
| 4 |
Beauch, Bryson J (7)
|
MIBC |
50.10 |
46.37 |
-3.73 |
| 5 |
Simkins, Nathan D (8)
|
MIEGRA |
|
48.54 |
- |
| 6 |
Ledesma, Oscar A (8)
|
MIMLA |
47.85 |
49.63 |
1.78 |
| 7 |
Koldenhoven, Elliott G (8)
|
MIEGRA |
|
51.59 |
- |
| 8 |
Cooper, Noah E (8)
|
MIOLY |
|
53.58 |
- |
| 9 |
Tufford-McKey, Emmett C (7)
|
MIMLA |
58.63 |
53.85 |
-4.78 |
| 10 |
Levesque, Asher J (8)
|
MIMLA |
53.81 |
55.01 |
1.20 |
| 11 |
Highstreet, Shay B (8)
|
MIMLA |
58.82 |
57.91 |
-0.91 |