| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Williamson, Eloise E (11)
|
GUDADS |
29.03 |
27.51 |
-1.52 |
| 2 |
Cone, Victoria G (12)
|
GUDADS |
32.51 |
30.91 |
-1.60 |
| 3 |
Ceraudo, Ava E (12)
|
GUDADS |
32.61 |
31.87 |
-0.74 |
| 4 |
Pham, Avery L (11)
|
GUDADS |
31.45 |
31.93 |
0.48 |
| 5 |
Czelusta, Katie N (11)
|
GUDADS |
35.15 |
34.85 |
-0.30 |
| 6 |
Fischbach, Claire E (11)
|
GUDADS |
41.39 |
36.02 |
-5.37 |
| 7 |
Toro, Laura A (12)
|
GUDADS |
37.44 |
36.84 |
-0.60 |
| 8 |
Hoskovec, Anna E (12)
|
GUDADS |
39.97 |
36.96 |
-3.01 |
| 9 |
Lang, Liberty S (12)
|
GUDADS |
36.28 |
37.29 |
1.01 |
| 10 |
Clechenko, Lillian N (12)
|
GUDADS |
41.27 |
37.70 |
-3.57 |
| 11 |
Garcia-Lara, Valentina (12)
|
GUPFL |
|
41.68 |
- |
| 12 |
Sackash, Kaelyn M (12)
|
GUPFL |
|
46.09 |
- |
| 13 |
Sackash, Kellyn A (11)
|
GUPFL |
|
49.80 |
- |
|
Jayr, Anais N (12)
|
GUDADS |
34.34 |
NS |
- |
|
Dharap, Chinmayi M (12)
|
GUPFL |
|
NS |
- |