| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stapleton, Tallis M (7)
|
GUSHAC |
40.08 |
40.75 |
0.67 |
| 2 |
Sosa, Lyza A (7)
|
GUSHAC |
41.21 |
41.21 |
0.00 |
| 3 |
Lindberg, Ainsley E (7)
|
GUBTA |
43.09 |
44.59 |
1.50 |
| 4 |
Valchar, Addley P (7)
|
GUSHAC |
42.46 |
45.75 |
3.29 |
| 5 |
Casperson, Cara E (7)
|
GUBTA |
46.43 |
49.67 |
3.24 |
| 6 |
Partin, Fiona M (7)
|
GUUN-S |
52.30 |
50.88 |
-1.42 |
| 7 |
Tisdall, Reese H (7)
|
GURICE |
50.48 |
51.33 |
0.85 |
| 8 |
Martinez, Azul (7)
|
GUHCAP |
58.07 |
51.62 |
-6.45 |
| 9 |
Riffel, Gemma A (7)
|
GUSHAC |
49.69 |
51.67 |
1.98 |
| 10 |
Villarreal, Addyson G (7)
|
GUBTA |
53.40 |
53.20 |
-0.20 |
| 11 |
Rubio, Liliana (7)
|
GUHCAP |
1:01.41 |
55.16 |
-6.25 |
| 12 |
McManus, Ashlyn M (7)
|
GUPEAK |
56.01 |
58.05 |
2.04 |
| 13 |
Foertsch, Katherine E (7)
|
GURICE |
59.93 |
1:02.38 |
2.45 |
| 14 |
Caver, Laila O (7)
|
GUSPA |
1:03.77 |
1:03.01 |
-0.76 |
| 15 |
Phan, Tam-Anh L (7)
|
GUPEAK |
|
1:06.05 |
- |
| 16 |
Li, Jasmine L (7)
|
GUPEAK |
|
1:06.43 |
- |
| 17 |
Vinson, Kaeden G (7)
|
GUPEAK |
1:16.78 |
1:08.82 |
-7.96 |
| 18 |
Marceleno, Sofia (7)
|
GUHCAP |
1:05.02 |
1:09.74 |
4.72 |
| 19 |
Gu, Cathy * (7)
|
GUPEAK |
|
1:15.03 |
- |