| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Qiu, Annabel Y (10)
|
GU300 |
39.49 |
38.44 |
-1.05 |
| 2 |
Guglielmello, Anelle C (10)
|
GUUN-V |
38.55 |
38.76 |
0.21 |
| 3 |
Zhao, Serena (9)
|
GU300 |
44.38 |
39.78 |
-4.60 |
| 4 |
Riebel, Audrey M (10)
|
GUCFSC |
44.32 |
39.85 |
-4.47 |
| 5 |
Olivo, Cipriana E (10)
|
GUCFSC |
46.84 |
41.49 |
-5.35 |
| 6 |
Liu, Amelia (10)
|
GU300 |
|
42.44 |
- |
| 7 |
Putnam, Kate A (10)
|
GUCFSC |
45.61 |
43.29 |
-2.32 |
| 8 |
Hicks, Addison L (10)
|
GUCFSC |
44.50 |
43.45 |
-1.05 |
| 9 |
Whitelock, Lilliana J (10)
|
GUCFSC |
|
43.57 |
- |
| 10 |
Tran, Josie (10)
|
GUCFSC |
|
44.56 |
- |
| 11 |
Brunkhorst, Addison G (9)
|
GUCFSC |
45.25 |
45.39 |
0.14 |
| 12 |
Riebel, Natalie G (9)
|
GUCFSC |
47.90 |
47.57 |
-0.33 |
| 13 |
Bhakta, Amiyah M (10)
|
GUCFSC |
47.83 |
47.95 |
0.12 |
| 14 |
Vyzasatya, Tapassuma G (10)
|
GUUN-S |
49.03 |
49.12 |
0.09 |
| 15 |
Du, Danica (10)
|
GU300 |
55.40 |
49.44 |
-5.96 |
| 16 |
Fritsche, Peyton B (9)
|
GUCFSC |
|
49.62 |
- |
| 17 |
Krishnan, Mihika H (9)
|
GU300 |
1:00.76 |
50.17 |
-10.59 |
| 18 |
Mutschler, Lily K (10)
|
GUUN-C |
45.56 |
52.07 |
6.51 |
| 19 |
Machala, Kyndall (9)
|
GUEPRC |
1:01.56 |
58.21 |
-3.35 |
| 20 |
Zavala, Lauren C (9)
|
GUCFSC |
|
1:02.13 |
- |
| 21 |
Pena-Perez, Karla (10)
|
GUCFSC |
|
1:09.36 |
- |
| 22 |
Blackwood, Sienna E (9)
|
GUCFSC |
|
1:20.29 |
- |
|
Underwood, Ella K (9)
|
GUSPA |
1:02.95 |
DQ |
- |