| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lin, Vincent Y (12)
|
GU300 |
35.36 |
32.36 |
-3.00 |
| 2 |
Suarez, Cruz L (11)
|
GUVSST |
35.53 |
33.47 |
-2.06 |
| 3 |
Davis, Harry D (11)
|
GUVSST |
41.34 |
34.22 |
-7.12 |
| 4 |
Villabroza, Jonas (12)
|
GUVSST |
|
38.49 |
- |
| 5 |
Fontenette, Jackson J (11)
|
GUCFSC |
41.38 |
39.04 |
-2.34 |
| 6 |
Speedie, Adam J (11)
|
GUVSST |
42.78 |
39.07 |
-3.71 |
| 7 |
Calvert, Ethan J (12)
|
GUCFSC |
38.54 |
39.66 |
1.12 |
| 8 |
Tran, Minh H (11)
|
GUCFSC |
49.06 |
42.50 |
-6.56 |
| 9 |
Gonzalez, Randy JR (11)
|
GUEPRC |
45.77 |
43.98 |
-1.79 |
| 10 |
Verano, Vincent Jon A (12)
|
GUSPA |
45.85 |
44.53 |
-1.32 |
| 11 |
Herrera, Thepte A (11)
|
GU300 |
47.16 |
44.68 |
-2.48 |
| 11 |
Van Beljon, Joshua L (11)
|
GUVSST |
|
44.68 |
- |
| 13 |
Nord, Cole W (12)
|
GUCFSC |
|
46.08 |
- |
| 14 |
Verhelst, James K (11)
|
GUCFSC |
48.04 |
48.18 |
0.14 |
| 15 |
Du, Alan (12)
|
GU300 |
|
48.24 |
- |
| 16 |
Warren, Jackson C (11)
|
GUCFSC |
|
51.71 |
- |
|
Ochoa, Even K (11)
|
GUCFSC |
|
NS |
- |
|
Herron, Finn A (11)
|
GUVSST |
|
NS |
- |
|
Shirley, Acen C (11)
|
GUVSST |
44.12 |
NS |
- |
|
Hernandez, Nathan J (11)
|
GUCFSC |
|
NS |
- |