| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stapleton, Tallis M (7)
|
GUSHAC |
34.27 |
33.15 |
-1.12 |
| 2 |
Sosa, Lyza A (7)
|
GUSHAC |
37.24 |
35.57 |
-1.67 |
| 3 |
Lindberg, Ainsley E (7)
|
GUBTA |
39.64 |
38.81 |
-0.83 |
| 4 |
Valchar, Addley P (7)
|
GUSHAC |
37.94 |
39.02 |
1.08 |
| 5 |
Tisdall, Reese H (7)
|
GURICE |
41.40 |
39.71 |
-1.69 |
| 6 |
Riffel, Gemma A (7)
|
GUSHAC |
41.09 |
40.49 |
-0.60 |
| 7 |
Martinez, Azul (7)
|
GUHCAP |
42.85 |
41.66 |
-1.19 |
| 8 |
Casperson, Cara E (7)
|
GUBTA |
43.36 |
42.13 |
-1.23 |
| 9 |
Fontenot, Kalyssa M (7)
|
GUSHAC |
48.61 |
43.40 |
-5.21 |
| 10 |
Villarreal, Addyson G (7)
|
GUBTA |
45.02 |
44.32 |
-0.70 |
| 11 |
Partin, Fiona M (7)
|
GUUN-S |
49.40 |
44.64 |
-4.76 |
| 12 |
Zhao, Jordana L (7)
|
GUPEAK |
46.27 |
46.59 |
0.32 |
| 13 |
Foertsch, Katherine E (7)
|
GURICE |
50.72 |
47.83 |
-2.89 |
| 14 |
Marceleno, Sofia (7)
|
GUHCAP |
1:06.90 |
55.17 |
-11.73 |
| 15 |
Vinson, Kaeden G (7)
|
GUPEAK |
1:09.01 |
57.86 |
-11.15 |
| 16 |
Caver, Laila O (7)
|
GUSPA |
1:03.46 |
57.97 |
-5.49 |
| 17 |
Phan, Tam-Anh L (7)
|
GUPEAK |
1:15.01 |
1:07.39 |
-7.62 |