| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Valchar, Addley P (7)
|
GUSHAC |
21.16 |
19.66 |
-1.50 |
| 2 |
Sosa, Lyza A (7)
|
GUSHAC |
20.04 |
19.76 |
-0.28 |
| 3 |
Lindberg, Ainsley E (7)
|
GUBTA |
20.10 |
20.66 |
0.56 |
| 4 |
Casperson, Cara E (7)
|
GUBTA |
21.72 |
22.70 |
0.98 |
| 5 |
Riffel, Gemma A (7)
|
GUSHAC |
21.51 |
22.76 |
1.25 |
| 6 |
Fontenot, Kalyssa M (7)
|
GUSHAC |
24.32 |
23.49 |
-0.83 |
| 7 |
Martinez, Azul (7)
|
GUHCAP |
23.40 |
23.94 |
0.54 |
| 8 |
Villarreal, Addyson G (7)
|
GUBTA |
24.61 |
24.11 |
-0.50 |
| 9 |
Partin, Fiona M (7)
|
GUUN-S |
28.23 |
24.78 |
-3.45 |
| 10 |
McManus, Ashlyn M (7)
|
GUPEAK |
24.71 |
27.62 |
2.91 |
| 11 |
Foertsch, Katherine E (7)
|
GURICE |
28.67 |
27.95 |
-0.72 |
| 12 |
Vinson, Kaeden G (7)
|
GUPEAK |
28.29 |
28.79 |
0.50 |
| 13 |
Caver, Laila O (7)
|
GUSPA |
26.10 |
28.97 |
2.87 |
| 14 |
Marceleno, Sofia (7)
|
GUHCAP |
32.35 |
30.10 |
-2.25 |
| 15 |
Phan, Tam-Anh L (7)
|
GUPEAK |
29.15 |
30.20 |
1.05 |