| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Helman, Larkin C (14)
|
GUDADS |
27.38 |
25.07 |
-2.31 |
| 2 |
Rautenbach, Ryan D (14)
|
GUTWST |
26.64 |
26.08 |
-0.56 |
| 3 |
O'Neil, Ian J (14)
|
GUDADS |
27.20 |
26.75 |
-0.45 |
| 4 |
Bose, Levi N (14)
|
STATAC |
27.98 |
26.85 |
-1.13 |
| 5 |
Karp, Gidean (13)
|
GUTWST |
29.42 |
27.92 |
-1.50 |
| 6 |
Guan, Timothy T (13)
|
STNTRO |
29.42 |
28.00 |
-1.42 |
| 7 |
O'Neil, Max E (14)
|
GUDADS |
28.20 |
28.10 |
-0.10 |
| 8 |
VanderVoort, Reid V (13)
|
GUDADS |
30.91 |
28.31 |
-2.60 |
| 9 |
Jones, Jackson C (14)
|
STATAC |
37.62 |
28.98 |
-8.64 |
| 10 |
Armstrong, Walter J (13)
|
STASC |
31.18 |
30.02 |
-1.16 |
| 11 |
Spencer, Samuel J (14)
|
GUKATY |
31.48 |
30.38 |
-1.10 |
| 12 |
Phillips, Seth M (14)
|
GUESA |
37.11 |
32.26 |
-4.85 |
| 13 |
Barley, Mack S (14)
|
STATAC |
35.40 |
34.10 |
-1.30 |
| 14 |
Esslemont, Jens T (13)
|
STNTRO |
32.50 |
34.69 |
2.19 |
| 15 |
Chow, Nehemiah C (13)
|
STASC |
|
35.49 |
- |
| 16 |
Ubanoski, Keegan M (13)
|
GUMAC |
44.29 |
37.33 |
-6.96 |
|
Mapes, Mitchell N (13)
|
GUKATY |
33.50 |
NS |
- |
|
Rodriguez Oregon, Iker E (14)
|
GUTWST |
|
NS |
- |