| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Koenst, Katelyn M (14)
|
GUSCAT |
2:24.51 |
2:21.27 |
-3.24 |
| 2 |
McWhorter, Rowan L (14)
|
GUTYES |
2:19.98 |
2:25.35 |
5.37 |
| 3 |
Robinson, Anaya P (14)
|
GUFRST |
2:23.52 |
2:28.14 |
4.62 |
| 4 |
Mao, Ella C (14)
|
GUSWIM |
2:30.72 |
2:30.10 |
-0.62 |
| 5 |
Wylie, Rebecca C (13)
|
GUSWIM |
2:31.16 |
2:30.97 |
-0.19 |
| 6 |
Goza, Natalie T (13)
|
GUSCAT |
2:30.77 |
2:34.82 |
4.05 |
| 7 |
Nash, Makenzie F (14)
|
GUSWIM |
2:37.50 |
2:36.99 |
-0.51 |
| 8 |
Canavan, Megan J (13)
|
GUESA |
2:45.52 |
2:39.68 |
-5.84 |
| 9 |
Koss, Ava G (14)
|
GUSWIM |
2:37.26 |
2:42.55 |
5.29 |
| 10 |
Salas Sanchez, Camila (13)
|
GUSST |
2:43.93 |
2:46.69 |
2.76 |
| 11 |
Salas Sanchez, Sofia (13)
|
GUSST |
2:46.35 |
2:51.09 |
4.74 |
| 12 |
Shockley, Arrie B (14)
|
GUNEHA |
|
2:53.95 |
- |
| 13 |
Alford, Brooklyn J (13)
|
GUSWIM |
|
2:54.26 |
- |
| 14 |
Adams, Lauren I (13)
|
GUOSAS |
|
2:54.50 |
- |
| 15 |
Tran, Leana T (13)
|
GUTYES |
3:06.15 |
2:57.48 |
-8.67 |
| 16 |
Garcia-Lara, Valentina (14)
|
GUPFL |
3:17.11 |
2:59.55 |
-17.56 |
| 17 |
Craig, MacKenzie M (14)
|
GUESA |
|
2:59.96 |
- |
| 18 |
Nieman, Michelle A (13)
|
GUBLST |
3:02.09 |
3:04.09 |
2.00 |
| 19 |
Laverde, Hannah M (13)
|
GULIFE |
|
3:04.90 |
- |
| 20 |
Niles, Karleigh F (14)
|
GUFRST |
|
3:05.26 |
- |
| 21 |
Sanders, Karianne K (14)
|
GUSWIM |
|
3:05.56 |
- |
| 22 |
Tompkins, Kathryn G (13)
|
GUFRST |
3:10.10 |
3:07.75 |
-2.35 |
| 23 |
Kilgore, Katelyn (13)
|
GUPFL |
|
3:24.40 |
- |
| 24 |
Kubeczka, Emma G (13)
|
GUSWIM |
|
3:32.33 |
- |
|
Chambers, Ella J (13)
|
GUTYES |
|
DQ |
- |
|
Nwaneri, Jordyn A (13)
|
GUTYES |
2:58.67 |
DQ |
- |
|
Vance, Katherine E (14)
|
GUSST |
2:26.21 |
NS |
- |