| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Green, Alexia A (16)
|
GUUN-B |
57.05 |
59.58 |
2.53 |
| 2 |
Lopez, Sarah (15)
|
GUSSAN |
59.87 |
1:00.99 |
1.12 |
| 3 |
Greeney, Paige M (15)
|
GUSSAN |
1:02.07 |
1:04.35 |
2.28 |
| 4 |
Fonseca, Abigail M (15)
|
GUSSAN |
1:02.74 |
1:04.65 |
1.91 |
| 5 |
Reeves, Emma M (16)
|
GUSCAT |
1:02.65 |
1:04.81 |
2.16 |
| 6 |
Caldwell, Isabella N (16)
|
GUUN-B |
|
1:05.11 |
- |
| 7 |
Tait, Reagan M (15)
|
GUSCAT |
1:05.78 |
1:06.42 |
0.64 |
| 8 |
Dozier, Chase M (16)
|
GUBTA |
1:03.72 |
1:06.47 |
2.75 |
| 9 |
Gallagher, Megan E (17)
|
GUUN-S |
|
1:07.19 |
- |
| 10 |
Kollatschny, Hannah E (16)
|
GUSSAN |
1:05.55 |
1:08.32 |
2.77 |
| 11 |
Docog, Gabrielle R (15)
|
GULSST |
1:07.47 |
1:08.53 |
1.06 |
| 12 |
O'Brien, Cinneide E (15)
|
GUUNAT |
|
1:13.78 |
- |
|
Lopez, Megan M (16)
|
GUBTA |
1:03.19 |
NS |
- |