| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Koenst, Katelyn M (14)
|
GUSCAT |
2:38.75 |
2:40.17 |
1.42 |
| 2 |
Fall, Madison A (13)
|
GUSSAN |
2:48.76 |
2:53.99 |
5.23 |
| 3 |
Solomon, Carlie H (14)
|
GUBTA |
|
2:55.61 |
- |
| 4 |
Hall, Taylor D (13)
|
GUUN-S |
3:06.81 |
2:56.97 |
-9.84 |
| 5 |
Twomey, Alexa J (13)
|
GUBTA |
2:53.28 |
2:57.10 |
3.82 |
| 6 |
Guillemette, Addison R (14)
|
GUBTA |
3:00.29 |
2:58.09 |
-2.20 |
| 7 |
Roesler, Morgan E (13)
|
GUBTA |
|
2:59.43 |
- |
| 8 |
Watson, Lizzie P (13)
|
GUBTA |
|
3:12.27 |
- |
| 9 |
Crenshaw, Michelle S (13)
|
GUSSAN |
3:09.62 |
3:14.73 |
5.11 |
| 10 |
Hall, Kendall M (13)
|
GUSSAN |
|
3:26.98 |
- |
| 11 |
Anderson, Allison P (13)
|
GUUN-S |
3:33.42 |
3:31.68 |
-1.74 |
| 12 |
Burrell, Flinn B (13)
|
GUOFSC |
|
3:39.07 |
- |
| 13 |
Contreras, Sofia I (13)
|
GUBTA |
|
3:40.48 |
- |
|
Ahrens, Carly M (14)
|
GUBTA |
2:34.00 |
NS |
- |