| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lieng, Natalie N (10)
|
GUUN-C |
|
44.80 |
- |
| 2 |
Fedoruk, Sarah E (10)
|
GUUN-V |
44.42 |
44.84 |
0.42 |
| 3 |
Chen, Gwyneth E (9)
|
GUEPRC |
44.06 |
45.33 |
1.27 |
| 4 |
Guglielmello, Anelle C (10)
|
GUUN-V |
51.58 |
46.20 |
-5.38 |
| 5 |
Kumar, Riya H (10)
|
GU300 |
52.87 |
51.63 |
-1.24 |
| 6 |
Crosby, Kathryn T (10)
|
GUCFSC |
52.22 |
52.75 |
0.53 |
| 7 |
Du, Danica (10)
|
GU300 |
52.14 |
53.28 |
1.14 |
| 8 |
Do, Julianne Q (10)
|
GUSPA |
1:01.65 |
55.06 |
-6.59 |
| 9 |
Espitia, Sofia (9)
|
GUVSST |
59.60 |
57.34 |
-2.26 |
| 10 |
Dermody, Abigail M (9)
|
GUCFSC |
|
58.15 |
- |
| 11 |
Fritsche, Peyton B (9)
|
GUCFSC |
|
59.21 |
- |
| 12 |
Machala, Kyndall (9)
|
GUEPRC |
1:05.55 |
1:01.20 |
-4.35 |
| 13 |
Vyzasatya, Tapassuma G (10)
|
GUUN-S |
1:06.78 |
1:02.54 |
-4.24 |
| 14 |
Brunkhorst, Addison G (9)
|
GUCFSC |
1:10.27 |
1:07.28 |
-2.99 |
| 15 |
Krishnan, Mihika H (9)
|
GU300 |
1:08.87 |
1:08.18 |
-0.69 |
|
Underwood, Ella K (9)
|
GUSPA |
1:27.27 |
DQ |
- |
|
Blackwood, Sienna E (9)
|
GUCFSC |
|
DQ |
- |