| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Partin, Fiona M (7)
|
GUSPA |
25.64 |
25.27 |
-0.37 |
| 2 |
Wiles, Sydney C (7)
|
GUTWST |
27.79 |
26.82 |
-0.97 |
| 3 |
FitzSullivan, Gabrielle S (7)
|
GUTWST |
29.45 |
27.23 |
-2.22 |
| 4 |
Zhao, Jordana L (7)
|
GUPEAK |
31.43 |
28.29 |
-3.14 |
| 5 |
Hess, Hyatt V (7)
|
GUPACK |
28.38 |
29.13 |
0.75 |
| 6 |
McManus, Ashlyn M (7)
|
GUPEAK |
30.58 |
30.06 |
-0.52 |
| 7 |
Perez, Emmalyn I (7)
|
GUBTA |
30.47 |
30.56 |
0.09 |
| 8 |
Heczey, Bella M (7)
|
GURICE |
|
31.04 |
- |
| 9 |
Steele, Makayla A (7)
|
GUSTA |
31.43 |
31.21 |
-0.22 |
| 10 |
Rosas Magos, Andrea (7)
|
GUTWST |
|
31.77 |
- |
| 11 |
Weikel, Trinity G (7)
|
GUESA |
31.88 |
31.84 |
-0.04 |
| 12 |
Brian, Hadley V (7)
|
GUESA |
31.10 |
32.53 |
1.43 |
| 13 |
Karbarz, Rachel F (7)
|
GUTEST |
|
33.12 |
- |
| 14 |
Wiesepape, Ashlyn L (7)
|
GUPEAK |
|
37.65 |
- |
| 15 |
Fiore, Launa S (7)
|
GUPACK |
|
38.03 |
- |
| 16 |
Frith, Piper A (7)
|
GUESA |
|
38.11 |
- |
| 17 |
Vaughan, Layla L (7)
|
GUPACK |
37.87 |
38.22 |
0.35 |
|
Gu, Cathy * (7)
|
GUPEAK |
37.88 |
DQ |
- |
|
Hypolite, Adeyn S (7)
|
GUPEAK |
|
DQ |
- |
|
Burns, Sydney E (7)
|
GUPEAK |
|
DQ |
- |
|
Ramirez-Davila, Emma L (7)
|
GUPACK |
59.17 |
DQ |
- |
|
Fritz, Amelia M (7)
|
GUBTA |
28.18 |
NS |
- |
|
Jafer, Dana A (7)
|
GUESA |
|
NS |
- |