| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Donnally, Keagan A (11)
|
GUSTA |
36.46 |
34.43 |
-2.03 |
| 2 |
Shirey, Gavin F (12)
|
GUAMBU |
36.69 |
35.73 |
-0.96 |
| 3 |
Grolemund, Liam J (12)
|
GUSSS |
38.46 |
36.46 |
-2.00 |
| 4 |
Foster, Christopher W (12)
|
GUSSS |
|
37.67 |
- |
| 5 |
Valure, Joseph (12)
|
GULIFE |
39.89 |
38.65 |
-1.24 |
| 6 |
Cogliatti, Gabriel K (11)
|
GULIFE |
|
41.48 |
- |
| 7 |
Kotelnikova, Dimitry (11)
|
GULIFE |
42.61 |
41.62 |
-0.99 |
| 8 |
Truan, Charlie G (11)
|
GULIFE |
|
42.75 |
- |
| 9 |
Rodriguez, Reece D (11)
|
GUUN-L |
49.56 |
44.13 |
-5.43 |
| 10 |
Antunez, Martin (12)
|
GULIFE |
46.97 |
44.99 |
-1.98 |
| 11 |
Chintalapally, Aryan R (12)
|
GULIFE |
|
45.61 |
- |
| 12 |
Yang, Jonathan (12)
|
GULIFE |
|
45.74 |
- |
| 13 |
Gillespie, John A (11)
|
GUSTA |
|
50.44 |
- |
| 14 |
Mimouni, Remi N (11)
|
GUTTST |
|
50.97 |
- |
| 15 |
Roth, Dylan C (11)
|
GULIFE |
50.22 |
53.46 |
3.24 |
| 16 |
Gutierrez, Daniel I (11)
|
GULIFE |
|
59.64 |
- |
|
Corning, Nicholas J (11)
|
GUAMBU |
|
NS |
- |
|
Lopez, Anthony (12)
|
GUAMBU |
34.46 |
NS |
- |