| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Alley, Katherine E (13)
|
GUESA |
28.43 |
27.73 |
-0.70 |
| 2 |
Gonzales, Chloe N (14)
|
GUNEHA |
29.34 |
29.09 |
-0.25 |
| 3 |
Walker, Rosemarri B (13)
|
GUESA |
28.92 |
29.37 |
0.45 |
| 4 |
Scott, Libby M (14)
|
GUESA |
31.43 |
29.50 |
-1.93 |
| 5 |
Verret, Ava G (13)
|
GUESA |
30.36 |
29.84 |
-0.52 |
| 6 |
Cayton, Abigail M (13)
|
GUESA |
29.51 |
29.94 |
0.43 |
| 7 |
Cotten, Kassidy A (13)
|
GUESA |
33.78 |
32.39 |
-1.39 |
| 8 |
Diaz-lazzarino, Valentina (13)
|
GUESA |
34.13 |
33.51 |
-0.62 |
| 9 |
Craig, MacKenzie M (13)
|
GUESA |
|
35.12 |
- |
| 10 |
Contreiras, Marina F (13)
|
GUESA |
37.72 |
35.37 |
-2.35 |
| 11 |
Luckett, Whitney D (13)
|
GUNEHA |
40.16 |
37.15 |
-3.01 |
| 12 |
Lerma, Nancy M (13)
|
GUBATS |
|
38.09 |
- |
|
Tatum, Lilian V (14)
|
GUESA |
27.16 |
NS |
- |