| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
George, Madison D (12)
|
GUESA |
29.04 |
29.28 |
0.24 |
| 2 |
Ackerman, Isabella R (11)
|
GUESA |
35.51 |
34.75 |
-0.76 |
| 3 |
Duncan, Grace K (11)
|
GUESA |
34.28 |
35.14 |
0.86 |
| 4 |
Canavan, Megan J (12)
|
GUESA |
34.56 |
35.42 |
0.86 |
| 5 |
Pink, Josephine G (11)
|
GUESA |
44.57 |
38.53 |
-6.04 |
| 6 |
Ivory, Ella M (11)
|
GUESA |
40.16 |
38.91 |
-1.25 |
| 7 |
Hejl, Taegen D (12)
|
GUUSA |
40.12 |
39.24 |
-0.88 |
| 8 |
Canalito, Eva A (12)
|
GUESA |
41.87 |
40.18 |
-1.69 |
| 9 |
Lastovica, Gabrielle M (12)
|
GUESA |
41.98 |
41.50 |
-0.48 |
| 10 |
Nguyen, Isabelle M (11)
|
GUUSA |
42.95 |
42.63 |
-0.32 |
| 11 |
Black, Janeva S (12)
|
GUUSA |
43.53 |
43.18 |
-0.35 |
| 12 |
Lino, Cassidy L (11)
|
GUESA |
|
1:05.65 |
- |
|
Taya, Anya Q (11)
|
GUESA |
|
DQ |
- |