| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Toler, Cassandra R (14)
|
GUSSS |
2:22.49 |
2:29.17 |
6.68 |
| 2 |
Reitenour, Danielle N (14)
|
GUBLST |
2:34.60 |
2:29.59 |
-5.01 |
| 3 |
Lopez, Catalina (14)
|
GUBLST |
2:35.42 |
2:33.52 |
-1.90 |
| 4 |
Higginbotham, Katherine M (14)
|
GUBLST |
2:35.12 |
2:34.44 |
-0.68 |
| 5 |
Rodriguez, Alyssandra I (14)
|
GUBLST |
2:33.27 |
2:34.47 |
1.20 |
| 6 |
Thorp, Madison J (14)
|
GUSSS |
2:39.55 |
2:35.21 |
-4.34 |
| 7 |
Martin, Abigail E (13)
|
GULIFE |
|
2:39.12 |
- |
| 7 |
Carrillo, Adeline A (14)
|
GUAMBU |
2:30.40 |
2:39.12 |
8.72 |
| 9 |
Zuniga, Reyna E (13)
|
GUBLST |
2:57.03 |
2:51.01 |
-6.02 |
| 10 |
Jenkins, Meredith R (13)
|
GUSTA |
3:09.58 |
2:53.28 |
-16.30 |
| 11 |
Smedstad, Hannah E (14)
|
GUSSS |
|
3:02.37 |
- |
| 12 |
Gillespie, Audrey N (13)
|
GUSTA |
3:20.54 |
3:14.56 |
-5.98 |
|
Pierce, Alexandra J (13)
|
GUAMBU |
2:55.08 |
DQ |
- |
|
Dawkins, Ava K (13)
|
GUSSS |
|
NS |
- |