| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bulseco, Kayla L (13)
|
GUSSS |
25.83 |
26.55 |
0.72 |
| 2 |
Troutman, Emily K (14)
|
GUSSS |
26.18 |
26.78 |
0.60 |
| 3 |
Huang, Anna J (13)
|
GUSSS |
27.85 |
28.08 |
0.23 |
| 4 |
Higginbotham, Katherine M (14)
|
GUBLST |
27.37 |
28.09 |
0.72 |
| 5 |
Dawlett, Niela T (13)
|
GUSSS |
28.26 |
28.13 |
-0.13 |
| 6 |
Carrillo, Adeline A (14)
|
GUAMBU |
27.56 |
28.42 |
0.86 |
| 7 |
Reitenour, Danielle N (14)
|
GUBLST |
27.77 |
28.58 |
0.81 |
| 8 |
Martin, Abigail E (13)
|
GULIFE |
|
28.61 |
- |
| 9 |
Prada, Sofia (13)
|
GULIFE |
30.49 |
28.89 |
-1.60 |
| 10 |
Ekhaese, Osatohame (13)
|
GUSSS |
30.67 |
29.88 |
-0.79 |
| 11 |
Block, Taliah M (13)
|
GUSTA |
31.19 |
29.97 |
-1.22 |
| 12 |
Thorp, Madison J (14)
|
GUSSS |
29.67 |
30.10 |
0.43 |
| 13 |
Cooper, Ashley M (13)
|
GULIFE |
|
30.51 |
- |
| 14 |
Jenkins, Meredith R (13)
|
GUSTA |
30.94 |
30.52 |
-0.42 |
| 15 |
Zuniga, Reyna E (13)
|
GUBLST |
31.60 |
31.38 |
-0.22 |
| 16 |
Pierce, Alexandra J (13)
|
GUAMBU |
31.99 |
31.44 |
-0.55 |
| 17 |
Lundquist, Kelley A (14)
|
GUSTA |
|
32.63 |
- |
| 18 |
Morse, Chloe L (13)
|
GULIFE |
|
38.86 |
- |
|
Dawkins, Ava K (13)
|
GUSSS |
29.52 |
NS |
- |
|
Aly, Mia Y (14)
|
GULIFE |
33.13 |
NS |
- |