| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gould, Serena M (16)
|
GUSTA |
51.23 |
53.10 |
1.87 |
| 2 |
Tilley, Erin E (16)
|
GULIFE |
51.84 |
57.42 |
5.58 |
| 3 |
Rohan, Micah J (15)
|
GUUN-R |
57.15 |
57.70 |
0.55 |
| 4 |
Shelburne, Catherine P (16)
|
GURICE |
57.77 |
59.60 |
1.83 |
| 5 |
Kriendler, Lauren E (16)
|
GUSWIM |
56.78 |
59.62 |
2.84 |
| 6 |
Long, Annika C (16)
|
GUBATS |
58.00 |
1:00.49 |
2.49 |
| 7 |
Noonan, Miranda B (15)
|
GUBATS |
1:00.60 |
1:02.53 |
1.93 |
| 8 |
Jenkins, Bryn H (15)
|
GUSTA |
1:02.84 |
1:02.60 |
-0.24 |
| 9 |
Plant, Callan S (16)
|
GUUN-S |
59.88 |
1:03.18 |
3.30 |
| 10 |
Wise, Lily I (15)
|
GUBATS |
1:02.69 |
1:04.11 |
1.42 |
| 11 |
Mason, Mia A (15)
|
GUSTA |
1:05.14 |
1:04.44 |
-0.70 |
| 12 |
Woodhouse, Hannah (15)
|
GURICE |
1:04.79 |
1:04.95 |
0.16 |
| 13 |
Hoffman, Mackenzie L (15)
|
GUSWIM |
1:01.99 |
1:05.04 |
3.05 |
| 14 |
Jensen, Olive Y (15)
|
GUBATS |
1:05.69 |
1:05.55 |
-0.14 |
| 15 |
Troeger, Martha A (16)
|
GUSTA |
1:05.19 |
1:06.05 |
0.86 |
| 16 |
Carroll, Jolee B (15)
|
GULIFE |
1:06.46 |
1:07.09 |
0.63 |
| 17 |
Ahn, Madeleine (15)
|
GURICE |
|
1:07.95 |
- |
| 18 |
Walker, Jaleah E (18)
|
GUBATS |
|
1:08.19 |
- |
| 19 |
Surguy, Raylee D (15)
|
GUSTA |
1:12.06 |
1:10.21 |
-1.85 |
| 20 |
Osorio, Britney W (16)
|
GUSWIM |
|
1:18.16 |
- |
|
Hudson, Catherine M (15)
|
GURICE |
|
NS |
- |
|
Buffington, Madeline R (15)
|
GUSWIM |
|
NS |
- |
|
Hildebrand, Devan N (17)
|
GUSWIM |
1:02.14 |
NS |
- |
|
Aly, Mia Y (15)
|
GULIFE |
1:14.64 |
NS |
- |