| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kolb, Diana E (16)
|
GUAMBU |
59.74 |
1:00.33 |
0.59 |
| 2 |
Duplantis, Angela G (17)
|
GUSTA |
1:04.37 |
1:04.14 |
-0.23 |
| 3 |
Bracher, Caitlyn R (16)
|
GUBLST |
1:04.86 |
1:05.25 |
0.39 |
| 4 |
Haines, Jaden M (15)
|
GUBLST |
1:11.19 |
1:07.69 |
-3.50 |
| 5 |
Jenkins, Bryn H (15)
|
GUSTA |
1:07.21 |
1:07.88 |
0.67 |
| 6 |
Eshelman, Samantha R (17)
|
GULIFE |
1:05.46 |
1:07.99 |
2.53 |
| 7 |
Mason, Mia (15)
|
GUSTA |
1:09.31 |
1:10.52 |
1.21 |
| 8 |
Troeger, Martha A (16)
|
GUSTA |
1:11.80 |
1:11.62 |
-0.18 |
| 9 |
Aguilera, Vianey (15)
|
GULIFE |
|
1:13.06 |
- |
| 10 |
Merritt, Evelyn M (15)
|
GUSSS |
1:13.76 |
1:13.50 |
-0.26 |
| 11 |
Fredregill, Sky B (16)
|
GUAMBU |
|
1:17.72 |
- |
| 12 |
Carroll, Jolee B (15)
|
GULIFE |
1:14.55 |
1:17.83 |
3.28 |
| 13 |
Valentine, Olivia C (17)
|
GUSSS |
1:28.22 |
1:22.15 |
-6.07 |
| 14 |
Pennartz, Lana R (15)
|
GUEPRC |
1:56.51 |
1:58.20 |
1.69 |
| 15 |
Rodriguez, Kristen L (16)
|
GUNOCH |
2:46.87 |
2:47.11 |
0.24 |
|
Wilburn, Hannah (16)
|
GUAMBU |
|
DQ |
- |
|
Miner, Aubree E (15)
|
GUSSS |
|
NS |
- |
|
Perez, Alydia M (15)
|
GUBLST |
1:17.79 |
NS |
- |
|
Tilley, Erin E (15)
|
GULIFE |
1:03.70 |
NS |
- |