| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hobbs, Lauren E (12)
|
NCMSA |
43.48 |
42.38 |
-1.10 |
| 2 |
Morisset, Karina M (12)
|
NCNMA |
41.64 |
42.47 |
0.83 |
| 3 |
Remella, Anvitha (12)
|
NCMSA |
42.55 |
42.63 |
0.08 |
| 4 |
Fisher, Ellison K (11)
|
NCMSA |
45.79 |
44.06 |
-1.73 |
| 5 |
Papatzanakis, Abigail J (11)
|
NCMSA |
45.97 |
44.79 |
-1.18 |
| 6 |
Driessen, Tarraleah L (12)
|
NCNMA |
46.28 |
45.40 |
-0.88 |
| 7 |
Lupinski, Gabrielle M (11)
|
NCMSA |
46.70 |
48.52 |
1.82 |
| 8 |
Hunt, Hailey A (11)
|
NCMSA |
49.99 |
49.55 |
-0.44 |
| 9 |
Connelly, Brooke A (12)
|
NCNMA |
49.71 |
49.57 |
-0.14 |
| 10 |
Murphy, Lauryn E (12)
|
NCMSA |
52.24 |
50.22 |
-2.02 |
| 11 |
Banks, Jordan H (12)
|
NCMSA |
|
50.70 |
- |
| 12 |
Drogaris, Katie A (11)
|
NCMSA |
|
50.79 |
- |
| 13 |
Beauchemin, Sarah G (12)
|
NCMSA |
|
50.85 |
- |
| 14 |
Canosa, Samantha M (12)
|
NCNMA |
52.02 |
51.00 |
-1.02 |
| 15 |
Dhir, Jia G (12)
|
NCMSA |
54.04 |
52.11 |
-1.93 |
| 16 |
Bolin, Jacquelyn O (12)
|
NCNMA |
53.56 |
52.93 |
-0.63 |
| 18 |
Schmitz, Madalyn E (12)
|
NCMSA |
56.42 |
55.70 |
-0.72 |
| 19 |
Hadebe, Wandisile S (12)
|
NCNMA |
56.42 |
57.00 |
0.58 |
| 20 |
Prayagkar, Mihika * (11)
|
NCMSA |
57.93 |
58.69 |
0.76 |
|
Sutsko, Kinsey L (11)
|
NCMSA |
50.80 |
DQ |
- |