| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Farrar, Tristan W (12)
|
AMPRA |
38.63 |
38.50 |
-0.13 |
| 2 |
Rupert, Gavin M (12)
|
AMPRA |
41.32 |
39.15 |
-2.17 |
| 3 |
Lavorini, Adrian (11)
|
AMBYAC |
39.19 |
39.22 |
0.03 |
| 4 |
Kolbe, Matthew J (12)
|
AMUN-R |
42.67 |
42.95 |
0.28 |
| 5 |
Aivalotis, Alexander C (11)
|
AMWAAC |
53.71 |
43.58 |
-10.13 |
| 6 |
Udumula, Venkata Sohan K (12)
|
AMSFAC |
47.09 |
44.51 |
-2.58 |
| 7 |
Verbytskyy, Andrey (11)
|
AMMOON |
45.00 |
44.81 |
-0.19 |
| 8 |
Vietmeier, Matthew A (12)
|
AMEFSC |
47.89 |
45.12 |
-2.77 |
| 9 |
Lowman, Samuel R (11)
|
AMWAAC |
43.99 |
45.25 |
1.26 |
| 10 |
Hickman, Troy J (12)
|
AMMOON |
50.18 |
45.81 |
-4.37 |
| 11 |
Burton, Matthew S (11)
|
AMMLAC |
57.33 |
46.58 |
-10.75 |
| 12 |
Jutca, Isaac A (11)
|
AMSFAC |
1:05.19 |
47.44 |
-17.75 |
| 13 |
Pidro, Gabriel J (12)
|
AMMLAC |
44.67 |
47.46 |
2.79 |
| 14 |
Lebak, Zachary D (12)
|
AMWAAC |
51.43 |
49.11 |
-2.32 |
| 15 |
Wang, Kevin (11)
|
AMSFAC |
53.46 |
49.59 |
-3.87 |
| 16 |
Kernan, Anthony J (12)
|
AMMOON |
1:16.63 |
53.80 |
-22.83 |
|
Walthour, James S (12)
|
AMWAAC |
1:30.98 |
DQ |
- |
|
Ye, Eric K (11)
|
AMWAAC |
1:20.34 |
DQ |
- |