| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Frank, Ryan M (12)
|
AMMOON |
31.42 |
27.81 |
-3.61 |
| 2 |
Farrar, Tristan W (12)
|
AMPRA |
29.84 |
28.84 |
-1.00 |
| 3 |
Zhu, Michael G (11)
|
AMMLAC |
29.73 |
29.26 |
-0.47 |
| 4 |
Sirbu, Daniel (12)
|
AMMOON |
31.08 |
30.34 |
-0.74 |
| 5 |
Thomas, William H (11)
|
AMMLAC |
31.17 |
30.78 |
-0.39 |
| 6 |
Aivalotis, Alexander C (11)
|
AMWAAC |
34.25 |
31.06 |
-3.19 |
| 7 |
Lowman, Samuel R (11)
|
AMWAAC |
31.09 |
31.45 |
0.36 |
| 8 |
Vietmeier, Matthew A (12)
|
AMEFSC |
31.19 |
31.51 |
0.32 |
| 9 |
Artman, Jake M (12)
|
AMSFAC |
32.85 |
32.17 |
-0.68 |
| 10 |
Freund, Brayden A (12)
|
AMMOON |
37.95 |
32.56 |
-5.39 |
| 11 |
Bliss, Hudson (11)
|
AMUN-R |
32.87 |
32.90 |
0.03 |
| 12 |
Wang, Kevin (11)
|
AMSFAC |
34.95 |
33.83 |
-1.12 |
| 13 |
Kolbe, Matthew J (12)
|
AMUN-R |
33.73 |
34.29 |
0.56 |
| 14 |
Pidro, Gabriel J (12)
|
AMMLAC |
36.62 |
34.88 |
-1.74 |
| 15 |
Verbytskyy, Andrey (11)
|
AMMOON |
36.03 |
35.00 |
-1.03 |
| 16 |
Jutca, Isaac A (11)
|
AMSFAC |
52.19 |
35.75 |
-16.44 |
| 17 |
Budzik, Jakob A (11)
|
AMWAAC |
40.00 |
36.33 |
-3.67 |
| 18 |
Hickman, Troy J (12)
|
AMMOON |
40.16 |
37.06 |
-3.10 |
| 19 |
Lebak, Zachary D (12)
|
AMWAAC |
37.67 |
38.17 |
0.50 |
| 20 |
Barton, Isaac (11)
|
AMUN-R |
43.03 |
40.27 |
-2.76 |
| 21 |
Kernan, Anthony J (12)
|
AMMOON |
44.61 |
41.91 |
-2.70 |
| 22 |
Walthour, James S (12)
|
AMWAAC |
1:00.87 |
44.96 |
-15.91 |
| 23 |
Quadrini, Carson A (11)
|
AMWAAC |
1:00.60 |
47.77 |
-12.83 |
| 24 |
Ye, Eric K (11)
|
AMWAAC |
1:05.05 |
1:05.56 |
0.51 |
|
Udumula, Venkata Sohan K (12)
|
AMSFAC |
34.33 |
NS |
- |
|
Gonugunta, Sandilya D (11)
|
AMSFAC |
1:08.19 |
NS |
- |
|
Burton, Matthew S (11)
|
AMMLAC |
39.29 |
NS |
- |
|
Goff, Nathaniel W (11)
|
AMUN-S |
53.59 |
NS |
- |