| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Waterson, Rebecca Q (15)
|
SNDART |
55.10 |
54.62 |
-0.48 |
| 2 |
Monroe, Teagan J (15)
|
CABUEN |
55.07 |
54.91 |
-0.16 |
| 3 |
O'Brien, Ashleen S (17)
|
PCDA |
54.84 |
56.84 |
2.00 |
| 4 |
Payne, Haily D (14)
|
IEVS |
56.40 |
56.89 |
0.49 |
| 5 |
Ryalls, Lindsey J (16)
|
NTTFA |
57.10 |
57.90 |
0.80 |
| 6 |
Duncan, Aurora R (15)
|
CAJCCW |
58.16 |
58.10 |
-0.06 |
| 7 |
Wolfe, Jaidyn E (17)
|
AZMAC |
57.13 |
58.12 |
0.99 |
| 8 |
Penrod, Allison M (17)
|
ILACAD |
1:02.97 |
59.01 |
-3.96 |
| 9 |
Blowers, Claudia L (16)
|
GUFCST |
1:00.68 |
59.04 |
-1.64 |
| 10 |
Rosenthal, Danielle V (16)
|
SINCA |
1:02.10 |
59.15 |
-2.95 |
| 11 |
Bolinger, Meredith J (16)
|
NTMTRO |
1:00.00 |
59.70 |
-0.30 |
| 12 |
Graham, Emily M (18)
|
ILWEST |
1:03.99 |
1:02.46 |
-1.53 |
|
Kobylak, Sylvia N (16)
|
ILWEST |
57.81 |
NS |
- |