| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Isaac, Jessie (11)
|
GUPLAT |
36.70 |
34.50 |
-2.20 |
| 2 |
Janak, Peyton E (12)
|
GUPLAT |
35.94 |
34.68 |
-1.26 |
| 3 |
Lorber, Lyla G (11)
|
GUESCA |
36.81 |
35.13 |
-1.68 |
| 4 |
Gunner, Chazlyn S (11)
|
GULIFE |
36.63 |
35.26 |
-1.37 |
| 5 |
Harris, Sydney J (11)
|
GUDADS |
35.45 |
36.29 |
0.84 |
| 6 |
Fraser, Chloe J (11)
|
GUTASC |
38.45 |
38.14 |
-0.31 |
| 6 |
Taeuber, Aliya L (12)
|
GUDADS |
39.24 |
38.14 |
-1.10 |
| 8 |
Baker, Lillian S (12)
|
GUDADS |
34.39 |
44.58 |
10.19 |
| 9 |
Kabele, Sophie G (12)
|
GUDADS |
32.40 |
45.33 |
12.93 |
| 10 |
McRee, Madison R (11)
|
GULIFE |
|
47.96 |
- |
| 11 |
Hornes, Emery C (11)
|
GUTASC |
31.85 |
49.90 |
18.05 |
|
Sachivichik, Alisa (11)
|
GUDADS |
50.30 |
NS |
- |
|
Lekic, Danica L (12)
|
GUDADS |
34.64 |
NS |
- |
|
Wilkinson, Olivia G (12)
|
GUDADS |
38.89 |
NS |
- |
|
Kolmel-Vieira, Isabella (11)
|
GUDADS |
37.48 |
NS |
- |
|
Cavazos, Sydney (12)
|
GULIFE |
36.47 |
NS |
- |
|
Harvin, Kacey E (11)
|
GUDADS |
34.93 |
NS |
- |
|
Glave, Kami T (11)
|
GUDADS |
33.84 |
NS |
- |
|
Ellisor, Brooke A (12)
|
GUOFSC |
30.73 |
NS |
- |
|
Fu, Zhihe (11)
|
GUDADS |
|
NS |
- |
|
Donkers, Kathelijne F (11)
|
GUDADS |
34.44 |
NS |
- |
|
Chen, Elaine Y (11)
|
GUDADS |
32.26 |
NS |
- |
|
Hebert, Sophie E (11)
|
GUBATS |
|
NS |
- |