| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clark, Quintin S (14)
|
NCGCY |
24.08 |
24.79 |
0.71 |
| 2 |
Cathey, Anderson M (13)
|
NCGCY |
24.58 |
25.09 |
0.51 |
| 3 |
Magrini, Alex M (14)
|
NCGCY |
24.85 |
25.17 |
0.32 |
| 4 |
Eskelund, Jesse E (13)
|
NCFAST |
26.67 |
25.53 |
-1.14 |
| 5 |
Fullagar, Keegan H (13)
|
NCGCY |
25.86 |
26.44 |
0.58 |
| 6 |
Williams, Jonathan P (14)
|
NCTYDE |
28.91 |
28.66 |
-0.25 |
| 7 |
Bivins, William D (14)
|
NCYBAC |
27.41 |
28.77 |
1.36 |
| 8 |
Newell, Chase C (14)
|
NCYBAC |
28.90 |
29.30 |
0.40 |
| 9 |
Worcester, William J (14)
|
NCYBAC |
32.24 |
31.94 |
-0.30 |
| 10 |
Bostic, Cassius L (13)
|
NCGCY |
55.99 |
32.25 |
-23.74 |
| 11 |
Kinneman, Gabriel W (13)
|
NCTYDE |
36.85 |
35.03 |
-1.82 |
| 12 |
McMillan, Cooper R (12)
|
NCYBAC |
39.74 |
38.07 |
-1.67 |