| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Najera, Aileen F (11)
|
NCHPSC |
32.90 |
32.38 |
-0.52 |
| 2 |
Doyle, Eva (11)
|
NCHPSC |
31.88 |
32.39 |
0.51 |
| 3 |
Schilling, Abigail R (11)
|
NCSGSA |
32.38 |
33.27 |
0.89 |
| 4 |
Wolff, Ameilia N (11)
|
NCSFSC |
36.16 |
33.83 |
-2.33 |
| 5 |
Day, Lauren E (11)
|
NCHPSC |
34.48 |
34.19 |
-0.29 |
| 6 |
Hartwick, Amelia L (11)
|
NCSGSA |
37.56 |
35.32 |
-2.24 |
| 7 |
Collins, Taylor E (11)
|
NCHPSC |
38.49 |
37.47 |
-1.02 |
| 8 |
O'Sullivan, Morgan S (11)
|
NCEAC |
37.31 |
37.58 |
0.27 |
| 9 |
Bateson, Halena M (11)
|
NCHPSC |
39.61 |
38.33 |
-1.28 |
| 10 |
Proco, Chloe A (11)
|
NCEAC |
40.00 |
38.40 |
-1.60 |
| 11 |
Grove, Kaye T (11)
|
NCSGSA |
38.91 |
38.53 |
-0.38 |
| 12 |
Kornatz, Maeve D (11)
|
NCEAC |
39.07 |
38.74 |
-0.33 |
| 13 |
Coleman, Rori K (11)
|
NCPAST |
40.87 |
43.86 |
2.99 |
| 14 |
Hutchison, Ella A (11)
|
NCPAST |
|
50.70 |
- |