| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Strasser, Morgan G (10)
|
NCSFSC |
41.90 |
40.71 |
-1.19 |
| 2 |
Wright, Lillian K (10)
|
NCEAC |
48.00 |
41.46 |
-6.54 |
| 3 |
Higgins, Harper A (10)
|
NCSFSC |
43.15 |
43.27 |
0.12 |
| 4 |
Fridinger, Cassidy H (10)
|
NCSFSC |
47.54 |
44.69 |
-2.85 |
| 5 |
Almond, Reece (10)
|
NCHPSC |
45.29 |
45.20 |
-0.09 |
| 6 |
Krumroy, Ainsley E (10)
|
NCSFSC |
46.24 |
46.66 |
0.42 |
| 7 |
Testa, Madeline C (10)
|
NCSGSA |
46.50 |
47.39 |
0.89 |
| 8 |
Holmes, Aliza M (10)
|
NCSFSC |
49.81 |
47.93 |
-1.88 |
| 9 |
Nelson, Zoe K (10)
|
NCSGSA |
54.75 |
49.13 |
-5.62 |
| 10 |
Knable, Lilliana M (10)
|
NCHPSC |
50.21 |
51.16 |
0.95 |
| 11 |
Matthis, Madison (10)
|
NCPAST |
53.43 |
51.62 |
-1.81 |
| 12 |
Granata, Ellisa J (10)
|
NCPAST |
54.13 |
51.84 |
-2.29 |
|
Spencer, Addison M (10)
|
NCHPSC |
39.13 |
39.15 |
0.02 |
|
Richardson, Sabina R (10)
|
NCSFSC |
1:00.89 |
DQ |
- |