| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Byrd, Audrey A (16)
|
GUSST |
24.87 |
25.48 |
0.61 |
| 3 |
Bernal Gomez, Paula A (16)
|
GUAGS |
26.63 |
26.32 |
-0.31 |
| 4 |
Dulay, Caitlin B (17)
|
GUSST |
27.09 |
27.30 |
0.21 |
| 5 |
Kroon, Gethsemane R (15)
|
GUSST |
26.74 |
27.34 |
0.60 |
| 6 |
Westwood, Julia K (16)
|
GUBTA |
26.44 |
27.49 |
1.05 |
| 7 |
Franklin, Mary Elizabeth (17)
|
GUAGS |
28.83 |
29.19 |
0.36 |
| 8 |
Barry, Elizabeth M (15)
|
GUNEHA |
30.04 |
29.39 |
-0.65 |
| 9 |
Knoerzer, Lexi L (15)
|
GUBTA |
29.53 |
30.16 |
0.63 |
| 10 |
Rodriguez, Ysabel C (15)
|
GUSST |
31.09 |
30.95 |
-0.14 |
| 11 |
Riddle, Allia M (15)
|
GUAGS |
33.77 |
31.85 |
-1.92 |
| 12 |
Kusler, Kaitlyn M (15)
|
GUBTA |
31.94 |
32.22 |
0.28 |
| 13 |
Garrou, Hannah N (15)
|
GUBTA |
|
37.35 |
- |
|
Surley, Abigail G (16)
|
GUAGS |
25.92 |
NS |
- |
|
Baggett, Emily G (16)
|
GUBTA |
27.71 |
NS |
- |