| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Figueredo-Weiser, Lukas (10)
|
GUVSST |
39.95 |
38.82 |
-1.13 |
| 2 |
Suarez, Cruz L (10)
|
GUVSST |
39.37 |
38.97 |
-0.40 |
| 3 |
McNames, Owen J (9)
|
GUPACE |
41.66 |
40.47 |
-1.19 |
| 4 |
Zhao, Johnathan M (10)
|
GUPACK |
41.53 |
40.87 |
-0.66 |
| 5 |
Armada, Adrian S (10)
|
GUPACK |
45.96 |
42.84 |
-3.12 |
| 6 |
Wright, Matthew S (10)
|
GUPACK |
45.62 |
44.82 |
-0.80 |
| 7 |
Carter, Matthew N (10)
|
GUVSST |
49.13 |
45.25 |
-3.88 |
| 8 |
Combs, Parker H (10)
|
GUSST |
47.48 |
46.24 |
-1.24 |
| 9 |
Kuebler, Ethan J (9)
|
GUPACK |
46.97 |
47.21 |
0.24 |
| 10 |
Revelas, Nicholas W (9)
|
GUVSST |
47.71 |
47.34 |
-0.37 |
| 11 |
Speedie, Adam J (10)
|
GUVSST |
48.27 |
48.47 |
0.20 |
| 12 |
Shah, Jonathan K (9)
|
GUPACK |
47.99 |
48.67 |
0.68 |
| 13 |
Zhang, Haoyang A (9)
|
GUPACK |
51.06 |
50.03 |
-1.03 |
| 14 |
Hess, Hagan W (9)
|
GUPACK |
51.40 |
50.97 |
-0.43 |
| 15 |
Nguyen, Ethan M (9)
|
GUUSA |
51.86 |
53.86 |
2.00 |
| 16 |
Hamm, Preston R (10)
|
GUPACK |
53.23 |
54.21 |
0.98 |
| 17 |
Roberts, Karim M (10)
|
GUPACK |
50.13 |
54.54 |
4.41 |
| 18 |
Wildermuth, Benjamin R (10)
|
GUPACK |
56.31 |
56.71 |
0.40 |
| 19 |
Larson-Hendricks, Anders R (10)
|
GUPACK |
1:04.54 |
56.77 |
-7.77 |
| 20 |
Rost, Marco G (10)
|
GUPACK |
56.26 |
57.04 |
0.78 |
| 21 |
Stansifer, Ty C (9)
|
GUPACK |
58.18 |
57.26 |
-0.92 |
| 22 |
Decena, Joshua Y (9)
|
GUPACK |
|
1:01.83 |
- |
| 23 |
Nguyen, Clayton T (9)
|
GUUSA |
1:06.07 |
1:01.85 |
-4.22 |
|
Zhu, Harrison L (10)
|
GUPACK |
1:00.86 |
DQ |
- |
|
Denton, Owen T (10)
|
GUPACK |
52.69 |
DQ |
- |