| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Longbottom, Charlotte V (16)
|
GUUN-P |
2:24.48 |
2:28.49 |
4.01 |
| 2 |
Talbot, Jayln K (17)
|
GUPACK |
2:39.90 |
2:38.28 |
-1.62 |
| 3 |
Gottfried, Charlotte A (15)
|
GUPACK |
2:38.58 |
2:40.39 |
1.81 |
| 4 |
Neciosup, Alexandra (16)
|
GUPACK |
2:35.41 |
2:41.03 |
5.62 |
| 5 |
Spencer, Alicia J (17)
|
GUPACK |
2:42.48 |
2:46.73 |
4.25 |
| 6 |
Revelas, Lily T (16)
|
GUPACK |
2:51.97 |
2:49.30 |
-2.67 |
| 7 |
Baldwin, Lindsay K (17)
|
GUESCA |
2:47.03 |
2:50.97 |
3.94 |
| 8 |
Wise, Shayna M (16)
|
GUTASC |
3:03.47 |
2:51.35 |
-12.12 |
| 9 |
Crawford, Cameron R (15)
|
GUPACK |
2:49.96 |
2:53.04 |
3.08 |
| 10 |
Martinez, Savannah B (16)
|
GUPACK |
3:09.57 |
2:53.36 |
-16.21 |
| 11 |
Tebo, Natalie N (17)
|
GUPACK |
3:00.99 |
2:56.64 |
-4.35 |
| 12 |
Molinaro, Mikaela G (15)
|
GUPACK |
2:53.01 |
2:57.70 |
4.69 |
| 13 |
Olson, Kyleigh E (15)
|
GUTASC |
2:46.21 |
2:57.77 |
11.56 |
| 14 |
Brady, Elise B (15)
|
GUPACK |
3:13.19 |
3:09.39 |
-3.80 |