| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Powell, Jessica B (13)
|
GUPACK |
1:03.67 |
1:04.12 |
0.45 |
| 2 |
Perales, Hailey L (13)
|
GUSST |
1:05.26 |
1:07.60 |
2.34 |
| 3 |
Griffin, Kennedy A (14)
|
GUPACK |
1:08.74 |
1:10.96 |
2.22 |
| 4 |
Reese, Laura M (13)
|
GUPACE |
1:06.73 |
1:13.29 |
6.56 |
| 5 |
Bourgeois-Solomon, Sovereign (14)
|
GUPACE |
1:13.13 |
1:14.94 |
1.81 |
| 6 |
Stepanik, Serenity A (13)
|
GUPACK |
1:14.04 |
1:15.52 |
1.48 |
| 7 |
Abrego, Kaylen J (14)
|
GUPACK |
1:13.70 |
1:17.17 |
3.47 |
| 8 |
Lindsey, Kyla J (14)
|
GUPACE |
1:16.89 |
1:19.59 |
2.70 |
| 9 |
Ryan, Alyssa Q (14)
|
GUPACE |
1:39.05 |
1:24.30 |
-14.75 |
| 10 |
Garcia, Victoria (13)
|
GUUSA |
1:31.53 |
1:24.81 |
-6.72 |
| 11 |
Williams, Kennedy L (14)
|
GUPACK |
1:27.58 |
1:26.00 |
-1.58 |
| 12 |
Green, Emily E (14)
|
GUPACE |
|
1:26.23 |
- |
| 13 |
Lester, Courtney A (13)
|
GUPACK |
1:41.31 |
1:34.29 |
-7.02 |
| 14 |
Estrello, Maranda L (14)
|
GUPACK |
|
1:59.18 |
- |