| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
English, Penelope L (10)
|
MAFCA |
43.01 |
42.09 |
-0.92 |
| 2 |
Walsh, Aivleen E (10)
|
MAYY |
|
42.28 |
- |
| 3 |
Dennis, Laken B (10)
|
MAMARA |
|
42.82 |
- |
| 4 |
Poon, Jaime C (10)
|
MACBST |
|
43.80 |
- |
| 5 |
Miller, Brianna E (10)
|
MAYY |
43.98 |
43.98 |
0.00 |
| 6 |
Jung, Seo Y (10)
|
MACBST |
|
44.14 |
- |
| 7 |
Williams, Lydia E (10)
|
MAYY |
42.78 |
44.60 |
1.82 |
| 8 |
Caron, Mackenzie C (10)
|
MAGMAC |
|
45.47 |
- |
| 8 |
Smallwood, Charlotte R (9)
|
MATRI |
|
45.47 |
- |
| 10 |
Landis, Dru K (9)
|
MAMARA |
|
45.72 |
- |
| 11 |
Kalmanowicz, Lauren G (9)
|
MAYY |
44.58 |
46.14 |
1.56 |
| 12 |
Moore, Violet E (10)
|
MAFCA |
46.32 |
46.52 |
0.20 |
| 13 |
Bell, Sofia R (10)
|
MACHEL |
|
46.90 |
- |
| 14 |
Brown, Anelia B (10)
|
MAFCA |
48.88 |
47.53 |
-1.35 |
| 15 |
Caron, Camryn M (9)
|
MAGMAC |
|
47.60 |
- |
| 16 |
Majewski, Anna C (9)
|
MACBST |
|
47.61 |
- |
| 17 |
Turner, Hannah M (10)
|
MAEPAC |
48.43 |
47.72 |
-0.71 |
| 18 |
Strader, Josephine E (10)
|
MAUN |
|
48.08 |
- |
| 19 |
Boyle, Jillian L (9)
|
MACBST |
|
48.57 |
- |
| 20 |
Xiong, Iris C (10)
|
MAFCA |
|
49.95 |
- |
| 21 |
Wolpert, Makenzie P (10)
|
MAMARA |
|
50.26 |
- |
| 22 |
Von Andreae, Elise m (9)
|
MAFCA |
57.07 |
51.25 |
-5.82 |
| 23 |
Deeter, Kaitlin C (10)
|
MACBST |
|
51.74 |
- |
| 24 |
Smith, Sophie S (9)
|
MAFCA |
54.28 |
53.53 |
-0.75 |
| 25 |
Shea, Elizabeth A (9)
|
MAFCA |
54.49 |
53.63 |
-0.86 |
| 26 |
Duranton, Natalia M (9)
|
MAFCA |
54.28 |
54.12 |
-0.16 |
| 27 |
Perlis, Gabrielle A (9)
|
MAFCA |
55.09 |
54.16 |
-0.93 |
| 28 |
Satheesh, Varunikha M (10)
|
MACBST |
|
54.97 |
- |
| 29 |
Marks, Hannah G (9)
|
MAMARA |
|
56.28 |
- |
| 30 |
Silot, Kimberly Juvi P (9)
|
MAEPAC |
|
57.45 |
- |
| 31 |
Boughanmi, Tasneem M (10)
|
MAEPAC |
|
1:01.15 |
- |
| 32 |
Laabs, Victoria M (10)
|
MAFCA |
1:00.32 |
1:04.13 |
3.81 |
| 33 |
Zhang, Claire R (9)
|
MAFCA |
|
1:07.87 |
- |
| 34 |
Swift, Emmaleigh N (9)
|
MAMARA |
|
1:09.89 |
- |