| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
D'Ariano, Landon N (10)
|
MASSC |
2:43.37 |
2:42.20 |
-1.17 |
| 2 |
Zagaceta, Magill M (9)
|
MAHAC |
2:48.33 |
2:44.55 |
-3.78 |
| 3 |
Zhou, Andrew R (10)
|
MAPWAC |
2:49.83 |
2:49.08 |
-0.75 |
| 4 |
Rees, Jonah A (10)
|
MAYY |
2:51.82 |
2:51.17 |
-0.65 |
| 5 |
Potts, Mason W (10)
|
MANPAC |
2:52.65 |
2:51.41 |
-1.24 |
| 6 |
Whitaker, Brock H (10)
|
MASSC |
2:55.62 |
2:51.84 |
-3.78 |
| 7 |
Henry, Christian J (9)
|
MAYY |
2:59.15 |
2:57.31 |
-1.84 |
| 8 |
Chen, Conan C (9)
|
MARAD |
3:06.03 |
2:57.85 |
-8.18 |
| 9 |
Pittala, Sritan (10)
|
MAUN-0 |
3:06.68 |
2:58.80 |
-7.88 |
| 10 |
Woren, Joshua L (10)
|
MACVAC |
3:06.42 |
3:00.36 |
-6.06 |
| 11 |
Mortellite, Anthony M (10)
|
MAUN-0 |
3:00.55 |
3:00.54 |
-0.01 |
| 12 |
Haney, James (10)
|
MAPVA |
3:07.11 |
3:01.25 |
-5.86 |
| 13 |
Chiu, Brandon (9)
|
MAFSS |
3:05.60 |
3:02.20 |
-3.40 |
| 14 |
Pervall, Ian C (10)
|
MADCAA |
3:03.90 |
3:02.70 |
-1.20 |
| 15 |
Vradinskiy, Sebastian (8)
|
MATHY |
3:02.75 |
3:03.27 |
0.52 |
| 16 |
Chang, Jonathan H (10)
|
MAWSY |
3:04.13 |
3:03.70 |
-0.43 |
| 17 |
Stewart, Jalen C (9)
|
MAEAAC |
3:12.07 |
3:04.05 |
-8.02 |
| 18 |
Grega, Alexander B (10)
|
MAYY |
3:09.31 |
3:05.98 |
-3.33 |
| 19 |
Hang, Kai V (10)
|
MAUPY |
3:02.70 |
3:06.10 |
3.40 |
| 20 |
Derivaux, Charles H (10)
|
MAJW |
2:47.48 |
3:09.69 |
22.21 |
| 21 |
Scholer, Richard Z (10)
|
MACBST |
3:13.32 |
3:09.95 |
-3.37 |
| 22 |
Almodovar, Jayden J (10)
|
MAMARA |
3:10.44 |
3:10.29 |
-0.15 |
| 23 |
Tsaturyan, Alec (10)
|
MAPWAC |
3:13.02 |
3:10.64 |
-2.38 |
| 24 |
Boratto, Evan A (9)
|
MAUDAC |
3:14.55 |
3:11.85 |
-2.70 |
| 25 |
Estep, Derek T (9)
|
MASHIP |
3:13.16 |
3:13.04 |
-0.12 |
| 26 |
Fritz, Owen P (10)
|
MANPAC |
3:14.00 |
3:13.72 |
-0.28 |
| 27 |
Ferker, Joshua E (9)
|
MALMOR |
2:41.97 |
3:15.83 |
33.86 |
| 28 |
Petrunich, Reece (10)
|
MAWEST |
3:10.57 |
3:18.49 |
7.92 |