| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wagner, Lauren M (17)
|
PVRY |
28.26 |
27.73 |
-0.53 |
| 2 |
Kirby, Ashley D (17)
|
PVRY |
28.87 |
28.31 |
-0.56 |
| 3 |
Pimentel, Amelia J (16)
|
PVRY |
29.51 |
28.71 |
-0.80 |
| 4 |
Romanowski, Leah M (13)
|
MDHFY |
29.32 |
30.09 |
0.77 |
| 5 |
Rucinskas, Gabrielle (16)
|
PVRY |
|
30.10 |
- |
| 6 |
Wagner, Amanda G (13)
|
PVRY |
30.98 |
30.78 |
-0.20 |
| 7 |
Zack, Grace L (17)
|
PVPAC |
|
31.62 |
- |
| 8 |
Shah, Amara U (15)
|
PVPAC |
|
32.06 |
- |
| 9 |
Jones, Shayla C (17)
|
PVPAC |
|
33.01 |
- |
| 10 |
DaRif, Lilly A (15)
|
PVPAC |
|
33.20 |
- |
| 11 |
Monarez, Isabella M (13)
|
PVASA |
33.60 |
34.39 |
0.79 |
| 12 |
Romanowski, Ashley A (13)
|
MDHFY |
34.47 |
34.95 |
0.48 |
| 13 |
Manela, Joanna R (13)
|
PVPAC |
41.77 |
35.55 |
-6.22 |
| 14 |
Golba, Brooke T (13)
|
MDHFY |
35.59 |
36.05 |
0.46 |
| 15 |
Hutchcroft, Haley A (14)
|
PVPAC |
36.07 |
38.10 |
2.03 |
| 16 |
DaRif, Addyson R (14)
|
PVPAC |
48.69 |
38.68 |
-10.01 |
| 17 |
Monroe, Devin S (14)
|
PVPAC |
54.57 |
49.79 |
-4.78 |