| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Campbell, Sydney K (8)
|
PVMSSC |
35.35 |
34.71 |
-0.64 |
| 2 |
Kim, Eurie (9)
|
PVMAC |
39.19 |
37.35 |
-1.84 |
| 3 |
Moyer, Kayli R (9)
|
MDHFY |
44.87 |
42.95 |
-1.92 |
| 4 |
Georgiou, Maria Olympia A (8)
|
PVJFD |
47.88 |
47.35 |
-0.53 |
| 5 |
Keating, Hannah M (8)
|
PVTIBU |
1:00.22 |
47.90 |
-12.32 |
| 6 |
Pemberton, Morgan L (9)
|
PVTIBU |
|
50.10 |
- |
| 7 |
Charles-Balthazar, Danariell (9)
|
PVNCAP |
1:06.83 |
51.29 |
-15.54 |
| 8 |
Whiteman, Samantha R (9)
|
PVFAST |
1:03.53 |
52.40 |
-11.13 |
| 9 |
Oram, Avery B (9)
|
PVJFD |
58.22 |
52.81 |
-5.41 |
| 10 |
Chong, Finley E (8)
|
PVTIBU |
1:00.49 |
56.32 |
-4.17 |
| 11 |
Smith, Camille L (9)
|
PVFAST |
|
58.60 |
- |
| 12 |
Brooks, Sydney (8)
|
PVHEAL |
|
59.16 |
- |
| 13 |
Parham, Chloe M (9)
|
PVNCAP |
1:07.25 |
1:05.21 |
-2.04 |
| 14 |
Biggs, Angelina O (8)
|
PVTIBU |
|
1:07.32 |
- |
| 15 |
Usquiano, Genesis D (9)
|
PVMSSC |
1:04.34 |
1:14.08 |
9.74 |
|
Ma, Haile T (9)
|
PVJFD |
|
DQ |
- |
|
Goodman, Ava D (8)
|
PVFAST |
|
DQ |
- |