| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shockley, Lola M (18)
|
MDHFY |
26.07 |
26.80 |
0.73 |
| 2 |
Zack, Grace L (18)
|
PVPAC |
28.17 |
28.28 |
0.11 |
| 3 |
Hendershot, Kelsi A (13)
|
PVPAC |
29.07 |
28.74 |
-0.33 |
| 4 |
Nelson, Ella S (15)
|
PVPAC |
28.35 |
28.86 |
0.51 |
| 5 |
Romanowski, Ashley A (14)
|
MDHFY |
28.04 |
28.90 |
0.86 |
| 6 |
Acevedo, Zoie M (15)
|
PVERSC |
|
29.89 |
- |
| 7 |
Castonguay, Elizabeth A (17)
|
PVPAC |
30.02 |
29.97 |
-0.05 |
| 8 |
Fetters, Abigail J (15)
|
MDHFY |
31.08 |
30.15 |
-0.93 |
| 9 |
Rea, Katherine G (16)
|
PVPAC |
29.94 |
30.29 |
0.35 |
| 10 |
Sutton, Jasmine L (14)
|
MDHFY |
31.32 |
30.64 |
-0.68 |
| 11 |
Cowperthwaite, Abigail M (13)
|
PVPAC |
32.83 |
32.01 |
-0.82 |
| 12 |
Vaughn, Sarah A (15)
|
PVPAC |
33.90 |
34.04 |
0.14 |
| 13 |
Mensah, Destinie N (14)
|
PVPAC |
37.10 |
38.66 |
1.56 |
| 14 |
Truong, Anna N (13)
|
PVPAC |
42.68 |
42.92 |
0.24 |
| 15 |
Castonguay, Megan P (13)
|
PVPAC |
41.31 |
44.49 |
3.18 |
|
Kim, Abigail Y (13)
|
PVTRA |
38.27 |
NS |
- |
|
Garcia, Julia S (16)
|
PVPAC |
34.58 |
NS |
- |