| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thomas, Halle (8)
|
PVYASD |
|
24.60 |
- |
| 2 |
Bui, AN T (8)
|
PVMSSC |
26.93 |
25.00 |
-1.93 |
| 3 |
Schlieter, Eloise (8)
|
PVYASD |
29.14 |
25.94 |
-3.20 |
| 4 |
Guerrieri Brown, Giulia (8)
|
PVDCPR |
31.66 |
26.38 |
-5.28 |
| 5 |
Charles, Danarielle V (8)
|
PVDCPR |
31.44 |
28.19 |
-3.25 |
| 6 |
Caraballo, Emma E (8)
|
PVDCPR |
|
28.78 |
- |
| 7 |
Guhr, Kajsa J (8)
|
PVDCPR |
|
31.43 |
- |
| 8 |
Fox, Chloe (7)
|
PVYASD |
33.82 |
31.94 |
-1.88 |
| 9 |
Minalu, Kalkidan A (7)
|
PVDCPR |
38.34 |
33.09 |
-5.25 |
| 10 |
Beggiato, Eva M (8)
|
PVDCPR |
|
33.59 |
- |
| 11 |
Marshall, Isabella M (8)
|
PVYASD |
35.76 |
35.37 |
-0.39 |
| 12 |
Whiteman, Samantha R (8)
|
PVFAST |
52.47 |
36.23 |
-16.24 |
| 13 |
Bascope, Maya M (7)
|
PVMSSC |
45.13 |
37.25 |
-7.88 |