| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaston, Noah A (12)
|
PVNCAP |
27.19 |
27.84 |
0.65 |
| 2 |
Barbour, Alexander F (11)
|
PVNCAP |
30.18 |
30.59 |
0.41 |
| 3 |
McMahon, William N (12)
|
PVPAC |
32.57 |
31.78 |
-0.79 |
| 4 |
Songu-Mbriwa, Nathanael K (11)
|
PVNCAP |
33.78 |
34.47 |
0.69 |
| 5 |
Tchama, Eyram H (12)
|
PVNCAP |
39.18 |
35.72 |
-3.46 |
| 6 |
Choi, Justin S (11)
|
PVPAC |
37.82 |
37.65 |
-0.17 |
| 7 |
Lightfoot, William A (11)
|
PVERSC |
37.70 |
38.35 |
0.65 |
| 8 |
Nguyen, Kenny T (11)
|
PVPAC |
37.76 |
38.75 |
0.99 |
| 9 |
Dickerson Jr, Michael S (11)
|
PVNCAP |
36.45 |
39.41 |
2.96 |
| 10 |
Sutton, Asher D (11)
|
PVPAC |
43.42 |
48.25 |
4.83 |
| 11 |
Hayes, Marius V (11)
|
PVPGKS |
1:28.74 |
56.57 |
-32.17 |