| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sunjaya, Viona K (10)
|
PVRMSC |
43.78 |
42.95 |
-0.83 |
| 2 |
Feltrin, Isadora (10)
|
PVRMSC |
45.13 |
44.38 |
-0.75 |
| 3 |
Ivanovskaya, Taisiya (10)
|
PVRMSC |
46.96 |
46.18 |
-0.78 |
| 4 |
Gravell, Kylie E (10)
|
PVRMSC |
46.44 |
47.15 |
0.71 |
| 5 |
Loftis, Jillian R (10)
|
PVRMSC |
47.48 |
47.99 |
0.51 |
| 6 |
Campbell, Sydney K (8)
|
PVMSSC |
45.99 |
49.34 |
3.35 |
| 7 |
Watson, Natalie V (9)
|
PVRMSC |
46.86 |
49.55 |
2.69 |
| 8 |
Day, Gabrielle M (10)
|
PVRMSC |
|
49.57 |
- |
| 9 |
Chang, Carleigh P (10)
|
PVRMSC |
49.65 |
49.91 |
0.26 |
| 10 |
Pearson, Eliana M (10)
|
PVNCAP |
50.30 |
51.92 |
1.62 |
| 11 |
Wang, Christine Y (9)
|
PVNCAP |
1:00.37 |
52.53 |
-7.84 |
| 12 |
Cutter, Maeve E (10)
|
PVRMSC |
56.49 |
53.56 |
-2.93 |
| 13 |
Awwad, Madelyn (10)
|
PVRMSC |
54.86 |
53.97 |
-0.89 |
| 14 |
Weiner, Isabelle G (10)
|
PVRMSC |
53.65 |
54.73 |
1.08 |
| 15 |
Newgen, Lanvy C (10)
|
PVRMSC |
53.88 |
54.74 |
0.86 |
| 16 |
Gaddis, Amisha M (10)
|
PVNCAP |
58.07 |
55.97 |
-2.10 |
| 17 |
Coggeshall, Corinne S (9)
|
PVRMSC |
55.71 |
57.53 |
1.82 |
| 18 |
Derrenbacher, Laney C (9)
|
PVNCAP |
59.92 |
57.66 |
-2.26 |
| 19 |
Hennelly, Samantha E (10)
|
PVRMSC |
1:03.10 |
58.69 |
-4.41 |
| 20 |
Hansel, Stella G (9)
|
PVTOLL |
1:01.57 |
1:00.67 |
-0.90 |
| 21 |
Xie, Leela N (10)
|
PVNCAP |
1:01.74 |
1:01.30 |
-0.44 |
| 22 |
Keating, Hannah M (8)
|
PVTIBU |
1:02.81 |
1:02.34 |
-0.47 |
| 23 |
Hartwell, Poppy A (9)
|
PVTOLL |
1:10.99 |
1:02.66 |
-8.33 |
| 24 |
Coble, Lily I (10)
|
PVPAC |
59.69 |
1:07.56 |
7.87 |
| 25 |
Achi, Fatimah Al-Zahra K (8)
|
PVPAC |
|
1:20.70 |
- |
|
Alexander, Mollie G (10)
|
PVNCAP |
1:08.92 |
DQ |
- |