| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Saarinen, Cecilia B (8)
|
PVPAC |
49.98 |
38.47 |
-11.51 |
| 2 |
Netherton, Scarlett M (7)
|
PVPAC |
46.10 |
39.19 |
-6.91 |
| 3 |
Lewis, Jillian A (8)
|
PVTIBU |
43.25 |
39.94 |
-3.31 |
| 4 |
Chong, Finley E (8)
|
PVTIBU |
39.64 |
40.23 |
0.59 |
| 5 |
Talberg, Natalie G (8)
|
PVLIFE |
48.15 |
41.30 |
-6.85 |
| 6 |
Keating, Hannah M (8)
|
PVTIBU |
53.53 |
43.52 |
-10.01 |
| 7 |
Batty, Leah M (8)
|
PVPAC |
48.85 |
45.83 |
-3.02 |
| 8 |
Evans, Khloe L (8)
|
PVPAC |
45.57 |
47.38 |
1.81 |
| 9 |
Peterson Delaney, Fiona R (8)
|
PVPAC |
46.05 |
48.43 |
2.38 |
| 10 |
Hughes, Emerson E (8)
|
PVPAC |
47.13 |
52.69 |
5.56 |
| 11 |
LaCarrubba, Adelina G (8)
|
PVPAC |
48.44 |
54.85 |
6.41 |
| 12 |
Ibrahim-Qayyum, Aniya Z (8)
|
PVTIBU |
54.82 |
56.08 |
1.26 |
| 13 |
Peterson Delaney, Ella J (8)
|
PVPAC |
52.16 |
57.13 |
4.97 |
| 14 |
Cooper, Ava (7)
|
PVPAC |
58.53 |
1:00.17 |
1.64 |
| 15 |
Martin, Alexandra B (8)
|
PVPGPR |
1:20.58 |
1:10.81 |
-9.77 |