| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Oehler, Morgan (17)
|
PVMSSC |
25.09 |
26.10 |
1.01 |
| 2 |
Heath, Lindsey D (18)
|
PVJFD |
26.97 |
27.01 |
0.04 |
| 3 |
Bielec, Jacqueline M (17)
|
PVFAST |
27.82 |
27.25 |
-0.57 |
| 4 |
Harrington, Hope N (16)
|
PVMSSC |
26.98 |
28.11 |
1.13 |
| 5 |
Yim, Eun-Ha (16)
|
MDHFY |
28.72 |
28.57 |
-0.15 |
| 6 |
Mavrikes, Julia C (16)
|
PVJFD |
27.97 |
28.69 |
0.72 |
| 7 |
Salazar, Anna S (17)
|
PVMSSC |
28.77 |
29.25 |
0.48 |
| 8 |
Willoughby, Wrayzene L (17)
|
PVDCPR |
28.38 |
29.61 |
1.23 |
| 9 |
McGrath, Jeannette T (16)
|
PVLCL |
30.58 |
30.23 |
-0.35 |
| 10 |
Hong, Amanda N (17)
|
PVMSSC |
28.54 |
30.55 |
2.01 |
|
Bloomer, Sky C (16)
|
PVDCPR |
27.99 |
NS |
- |
|
Chamorro-Beckenhauer, Irene (16)
|
PVDCPR |
30.66 |
NS |
- |
|
Jarman, Nancy (16)
|
PVDCPR |
30.82 |
NS |
- |
|
Sanabria, Sabrina (17)
|
PVDCPR |
31.57 |
NS |
- |
|
Wood, Kalyse T (16)
|
PVDCPR |
31.13 |
NS |
- |
|
Russell, Dhana K (16)
|
PVPGPR |
30.86 |
NS |
- |