| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gendron, Karina (13)
|
MAWEST |
2:37.36 |
2:33.15 |
-4.21 |
| 2 |
Dreibelbis, Sara E (13)
|
MDSPY |
2:40.62 |
2:34.65 |
-5.97 |
| 3 |
Helojoki, Haden K (14)
|
MAWEST |
2:38.70 |
2:37.09 |
-1.61 |
| 4 |
Hlasko, Paula A (14)
|
MDYCM |
2:39.42 |
2:37.63 |
-1.79 |
| 5 |
Otto, Cayla M (14)
|
MDFCY |
2:42.66 |
2:37.89 |
-4.77 |
| 6 |
Stoops, Anna M (14)
|
PVRY |
2:39.61 |
2:38.53 |
-1.08 |
| 7 |
Young, Kadence Y (14)
|
MAWEST |
2:38.35 |
2:38.81 |
0.46 |
| 8 |
Hyde, Zoe L (14)
|
MDYCM |
2:49.25 |
2:41.61 |
-7.64 |
| 9 |
Criswell, Katherine M (14)
|
PVRY |
3:01.41 |
2:42.39 |
-19.02 |
| 10 |
Preuett, Anna O (14)
|
MDCATY |
2:44.70 |
2:47.11 |
2.41 |
| 11 |
Rucinskas, Gabrielle (13)
|
PVRY |
2:43.58 |
2:49.62 |
6.04 |
| 12 |
Forstner, Nina (14)
|
MDYBCC |
3:05.00 |
2:51.69 |
-13.31 |
| 13 |
Magbulos, Jaime Ann H (13)
|
MDYCM |
2:55.85 |
2:53.92 |
-1.93 |
| 14 |
Xue, Holly Y (13)
|
PVRY |
3:00.56 |
2:55.62 |
-4.94 |
| 15 |
Clawson, Sarah F (14)
|
CECY |
3:06.76 |
2:58.95 |
-7.81 |
| 16 |
Cleveland, Abigail R (14)
|
MDSPY |
2:57.05 |
3:00.60 |
3.55 |
| 17 |
Shroff, Hannah (13)
|
MDYBCC |
2:59.32 |
3:01.44 |
2.12 |
| 18 |
Eagen, Brynne E (14)
|
MDFCY |
3:01.67 |
3:12.87 |
11.20 |
| 19 |
Ranasinghe, Sanjana (13)
|
MDFCY |
3:39.45 |
3:18.57 |
-20.88 |
| 20 |
Mariott, Annabella (14)
|
MDFCY |
3:26.34 |
3:22.70 |
-3.64 |