| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Depman, Megan A (10)
|
PVJFD |
2:39.87 |
2:32.68 |
-7.19 |
| 2 |
Zhou, Angel (10)
|
PVMSSC |
2:58.12 |
2:41.17 |
-16.95 |
| 3 |
Reinhardt, Jane R (10)
|
PVFISH |
2:49.57 |
2:42.12 |
-7.45 |
| 4 |
Stein, Abigail A (10)
|
PVFISH |
3:11.57 |
2:44.28 |
-27.29 |
| 5 |
Buxo, Alexis R (10)
|
PVPGPR |
3:22.18 |
2:46.73 |
-35.45 |
| 6 |
Williams, Jill C (9)
|
PVMACH |
3:00.21 |
2:50.93 |
-9.28 |
| 7 |
Hayward, Audrey I (9)
|
PVBWST |
2:47.10 |
2:52.61 |
5.51 |
| 8 |
Hooff, Olivia (9)
|
PVYASD |
3:06.01 |
3:00.27 |
-5.74 |
| 9 |
Jones, Sofia K (9)
|
PVFAST |
3:24.63 |
3:20.53 |
-4.10 |
| 10 |
Roberge, Connelly E (9)
|
PVMACH |
3:29.81 |
3:27.12 |
-2.69 |
| 11 |
Cortes-Rodriquez, Sofia (9)
|
PVYASD |
|
3:29.20 |
- |
| 12 |
Huddleston, Natalie T (10)
|
PVTIBU |
|
3:46.56 |
- |
| 13 |
Yuan, Grace S (9)
|
PVFISH |
|
4:00.02 |
- |
|
Choe, Lucy E (10)
|
PVLIFE |
|
NS |
- |
|
Cronin, Hailey M (10)
|
PVMACH |
2:39.95 |
NS |
- |
|
Williams, Maeve A (10)
|
PVMACH |
2:55.39 |
NS |
- |