| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hall, Jay-Colin S (12)
|
GULAP |
31.80 |
31.77 |
-0.03 |
| 2 |
Subramanya, Rishab (11)
|
GUHCAP |
34.91 |
33.30 |
-1.61 |
| 3 |
Macedo, Antonio D (11)
|
GUHCAP |
35.99 |
35.73 |
-0.26 |
| 4 |
Paelicke, Ian H (12)
|
GUSSS |
38.17 |
37.07 |
-1.10 |
| 5 |
Soto, Eduardo A (11)
|
GUCOOG |
36.24 |
37.27 |
1.03 |
| 6 |
Barrera, Juan S (12)
|
GUHCAP |
39.12 |
37.43 |
-1.69 |
| 7 |
Nielsen, Hayden T (11)
|
GUSHRK |
36.84 |
37.62 |
0.78 |
| 8 |
Lopez Turley, Alejandro D (12)
|
GUCOOG |
35.81 |
38.91 |
3.10 |
| 9 |
Morales, Sebastian A (12)
|
GUHCAP |
39.62 |
39.69 |
0.07 |
| 10 |
D'Onofrio, Cole (12)
|
GUSSS |
38.48 |
40.91 |
2.43 |
| 11 |
Stratton, Mac D (11)
|
GUCOOG |
41.45 |
41.19 |
-0.26 |
| 12 |
Leslie, Hayden V (12)
|
GUSSS |
42.67 |
41.84 |
-0.83 |
| 13 |
Bhakta, Vishaan K (11)
|
GUSHRK |
44.94 |
42.18 |
-2.76 |
| 14 |
Givens, Noah W (11)
|
GUSHRK |
45.20 |
44.24 |
-0.96 |
| 15 |
Comee, Vladimir L (11)
|
GUSSS |
47.25 |
45.95 |
-1.30 |
| 16 |
Guchhait, Archisman (11)
|
GUCOOG |
|
48.55 |
- |
| 17 |
Ganapathy, Auyush (11)
|
GUSHRK |
51.08 |
51.79 |
0.71 |
| 18 |
Mounts, Christian M (11)
|
GUTCAP |
53.06 |
54.70 |
1.64 |
| 19 |
Chen, David (11)
|
GUSSS |
|
58.53 |
- |
|
Remington, Jared D (11)
|
GUSHRK |
42.55 |
DQ |
- |