| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pheister, Robert E (8)
|
GUAQUA |
36.83 |
36.76 |
-0.07 |
| 2 |
Chen, Albert E (6)
|
GUEPRC |
1:06.46 |
41.61 |
-24.85 |
| 3 |
Vandertie, Crichton J (8)
|
GUAQUA |
|
43.30 |
- |
| 4 |
Sigler, Aiden N (8)
|
GUSHAC |
45.08 |
44.64 |
-0.44 |
| 5 |
Alfaro, Adrian (7)
|
GUHCAP |
|
45.05 |
- |
| 6 |
Williams, Emory A (8)
|
GUHCAP |
|
46.68 |
- |
| 7 |
Carr, Blake S (8)
|
GUSSS |
55.18 |
46.80 |
-8.38 |
| 8 |
Ellington, Ethan C (8)
|
GUSCAT |
|
47.63 |
- |
| 9 |
Williams, Ronin R (8)
|
GUSHAC |
48.61 |
47.65 |
-0.96 |
| 10 |
Donart, Patrick Ryan R (8)
|
GUSCAT |
|
50.52 |
- |
| 11 |
Maronge, Kellen P (8)
|
GUAQUA |
|
50.92 |
- |
| 12 |
Messenger, Ethan M (8)
|
GUSSS |
|
54.44 |
- |
| 13 |
Salinas, Carlos (8)
|
GUHCAP |
|
55.86 |
- |
| 14 |
Svahn, Andrew T (8)
|
GUAQUA |
|
59.70 |
- |
| 15 |
Davis, Jace A (8)
|
GUAQUA |
|
1:00.15 |
- |
| 16 |
Lambert, Braxton F (8)
|
GUGCST |
1:11.52 |
1:00.82 |
-10.70 |
| 17 |
Kim, Christian T (8)
|
GUSCAT |
|
1:01.66 |
- |
| 18 |
Perales, Makai J (8)
|
GUSSS |
|
1:10.65 |
- |
| 19 |
Donart, Dylan A (6)
|
GUSCAT |
|
1:22.17 |
- |
| 20 |
West, Justin N (6)
|
GUSSS |
|
1:22.86 |
- |
| 21 |
West, Jake A (6)
|
GUSSS |
|
2:02.36 |
- |