| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zagaceta, Magill M (9)
|
MAHAC |
45.18 |
40.39 |
-4.79 |
| 2 |
Hang, Kai V (10)
|
MAUPY |
51.28 |
43.09 |
-8.19 |
| 3 |
Woerner, Davis C (10)
|
MALCY |
43.37 |
45.44 |
2.07 |
| 4 |
Wu, Wade (10)
|
MABLUE |
38.98 |
45.95 |
6.97 |
| 5 |
Chang, Jonathan H (10)
|
MAWSY |
52.23 |
48.10 |
-4.13 |
| 6 |
Jackson, Brady (10)
|
MACC |
53.80 |
49.99 |
-3.81 |
| 7 |
Petrunich, Reece (10)
|
MAWEST |
44.45 |
50.51 |
6.06 |
| 8 |
Lehman, Zachary C (10)
|
MAPAAC |
53.78 |
50.81 |
-2.97 |
| 9 |
Estep, Derek T (9)
|
MASHIP |
|
51.24 |
- |
| 10 |
McCloskey, Nolan P (9)
|
MAUPY |
57.92 |
51.83 |
-6.09 |
| 11 |
Serfass, Caleb P (10)
|
MAHAC |
57.62 |
52.15 |
-5.47 |
| 12 |
Barone, Ryan (9)
|
MAWSY |
56.35 |
52.18 |
-4.17 |
| 13 |
Oliver, Donovan B (9)
|
MAPFY |
51.34 |
52.63 |
1.29 |
| 14 |
Lynch, Ren W (10)
|
MAWEST |
53.12 |
52.91 |
-0.21 |
| 15 |
Wingert, Markus J (9)
|
MAHAC |
52.33 |
53.75 |
1.42 |
| 16 |
Bu, Tianshi J (10)
|
MAHAC |
46.84 |
54.99 |
8.15 |
| 17 |
Kahwajy, Andrew F (10)
|
MDFSC |
57.15 |
55.13 |
-2.02 |
| 18 |
Van De Louw, Mathias (10)
|
MAHAC |
56.27 |
55.61 |
-0.66 |
| 19 |
Chappell, William C (9)
|
MAHAC |
51.99 |
55.94 |
3.95 |
| 20 |
Arar, Hasan E (10)
|
MABRY |
53.79 |
56.98 |
3.19 |
| 21 |
Hendershot, Carter R (10)
|
MACYA |
55.58 |
57.68 |
2.10 |
| 22 |
Lehan, Sean C (10)
|
MAHAC |
56.09 |
57.75 |
1.66 |
| 23 |
Jeffers, Issac M (10)
|
MAUPY |
1:03.61 |
58.10 |
-5.51 |
| 24 |
Lamison, Isaac W (10)
|
MACYA |
1:10.00 |
59.10 |
-10.90 |
| 25 |
Coffman, Cole C (10)
|
MABLUE |
1:02.38 |
1:00.20 |
-2.18 |
| 26 |
King, Brady C (9)
|
MAEMAC |
|
1:01.45 |
- |
| 27 |
Sheetz, Gavin P (9)
|
MAUN |
|
1:02.30 |
- |
| 28 |
Burnell, Luke D (9)
|
MAWEST |
58.21 |
1:02.75 |
4.54 |
| 29 |
Vlaeminck, Julian P (9)
|
MAEMAC |
|
1:05.68 |
- |
| 30 |
Elliott, Parker R (10)
|
MAUPY |
|
1:13.26 |
- |