| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carlson, Jaelle S (17)
|
NTMTRO |
26.08 |
27.13 |
1.05 |
| 2 |
Perna, Amie M (15)
|
NTESP |
30.09 |
28.07 |
-2.02 |
| 3 |
Reynolds, Lena I (13)
|
NTLTSD |
28.39 |
28.97 |
0.58 |
| 4 |
Beeson, Abigail D (15)
|
NTESP |
30.11 |
29.08 |
-1.03 |
| 5 |
Bogue, Caroline E (16)
|
NTTRAC |
28.85 |
29.74 |
0.89 |
| 6 |
Witte, Carissa R (13)
|
NTLTSD |
35.20 |
29.82 |
-5.38 |
| 7 |
Terburgh, Olivia R (16)
|
NTTRAC |
33.00 |
29.86 |
-3.14 |
| 8 |
Buenaventura, Tharyn H (13)
|
NTTRAC |
31.34 |
30.14 |
-1.20 |
| 9 |
Tsing, Alyssa E (17)
|
NTMARS |
|
31.07 |
- |
| 10 |
Burlingame, Ariana K (14)
|
NTESP |
32.12 |
31.47 |
-0.65 |
| 11 |
Lloyd, Kalynn N (14)
|
NTTRAC |
33.26 |
31.50 |
-1.76 |
| 12 |
Humphrey, Chloe E (16)
|
NTMARS |
|
31.54 |
- |
| 13 |
Irvin, Jadyn E (14)
|
NTESP |
30.31 |
32.29 |
1.98 |
| 14 |
Muse, Naomi E (14)
|
NTTRAC |
33.78 |
32.55 |
-1.23 |
| 15 |
Lemmert, Ellie C (13)
|
NTTRAC |
34.31 |
33.68 |
-0.63 |
| 16 |
Dangelmaier, Anja E (15)
|
NTMARS |
36.29 |
33.95 |
-2.34 |
| 17 |
Reed, Paige I (13)
|
NTESP |
40.02 |
34.76 |
-5.26 |
| 18 |
Bataraga, Rozalija (17)
|
NTMARS |
|
35.56 |
- |
| 19 |
Ellis, Caroline G (13)
|
NTESP |
36.36 |
35.70 |
-0.66 |
| 20 |
Delk, Rachel A (13)
|
NTMTRO |
35.73 |
36.14 |
0.41 |
|
O'Hara, Emrie L (14)
|
NTMTRO |
43.81 |
NS |
- |
|
Carter, Madilyn E (14)
|
NTMARS |
|
NS |
- |
|
Beeson, Hadley C (17)
|
NTESP |
|
NS |
- |